Yeast Two-hybrid Media Sets
Yeast Two-hybrid Media Sets
Brand: Takara Bio.
In stock
SKU
Yeast Two-hybrid Media Sets
The Yeast Media Set 2 Plus contains the media and supplements needed for yeast two-hybrid library screening with the Matchmaker Gold Yeast Two-Hybrid System (Cat. No. 630489). For the ready-to-go, preformulated pouches, just add water and autoclave according to the instructions provided in the Yeast Media Protocol-at-a-Glance: no measuring, mixing, or pH adjustments are required. Directions for the use of Aureobasidin A and X-alpha-Gal are provided in the Matchmaker Gold Yeast Two-Hybrid System User Manual.
Overview
- Complete media sets for two-hybrid screening
- Convenient; all you need for Matchmaker Gold systems in a single package
- Extra value; cheaper than purchasing the media separately
- Simply mix each pouch with 500 ml water and sterilize
The yeast two-hybrid principle
The yeast two-hybrid principle. A bait protein interacts with the GAL4 recognition sequence (or promoter) upstream of a reporter gene. Transcription of the reporter is activated when a prey protein containing the GAL4 transcriptional activation domain interacts with the bait.
Matchmaker Gold reporter genes
Matchmaker Gold reporter genes. Yeast strain Y2HGold expresses 4 genes from 3 separate GAL4-responsive promoters in response to protein-protein interactions.
Write Your Own Review
The yeast two-hybrid principle
The yeast two-hybrid principle. A bait protein interacts with the GAL4 recognition sequence (or promoter) upstream of a reporter gene. Transcription of the reporter is activated when a prey protein containing the GAL4 transcriptional activation domain interacts with the bait.
Matchmaker Gold reporter genes
Matchmaker Gold reporter genes. Yeast strain Y2HGold expresses 4 genes from 3 separate GAL4-responsive promoters in response to protein-protein interactions.
