Yeast minimal media/synthetic-defined (SD) premixes
Yeast minimal media/synthetic-defined (SD) premixes
Brand: Takara Bio.
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Yeast minimal media/synthetic-defined (SD) premixes
Overview
- Minimal media for yeast—conveniently premixed
- Large selection of dropout mixes
- No weighing, mixing, or pH adjustment required
- Simply mix each pouch with 500 ml water and sterilize
- Proven track record—15 years of yeast media sales
Applications
- Culturing auxotrophic yeast strains
- Yeast transformation and selection
- Yeast two-hybrid screening
- Yeast one-hybrid screening
Yeast minimal media and synthetic defined premixes (SD) come in highly convenient single-use pouches. Simply empty the content of the pouch into your media flask/bottle, add 0.5 L of dH2O, and sterilize. No weighing of individual supplements, combining, or pH adjustment is required.
With or without agar
All minimal yeast media pouch mixes are available either with agar or without (broth).
Minimal media
Synthetic defined (SD) broths and agar minimal media consist of a carbon source (glucose), a nitrogen source, and amino acids. In each particular SD mix, certain amino acids have been “dropped out”. For example, SD/-Leu/-Trp contains all essential amino acids except for leucine and tryptophan.
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