XhoI

XhoI

Brand: Takara Bio.
In stock
SKU
XhoI
Grouped product items
Product Name Size
XhoI
SKU: 1094A
5000 Units
XhoI
SKU: 1094B
25000 Units
XhoI (High Concentration)
SKU: 1094AH
5000 Units
XhoI (High Concentration)
SKU: 1094BH
25000 Units
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XhoI
XhoI

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XhoI restriction site:

C | TCGA G
G AGCT | C

Supplied buffer: H

Effect of DNA methylation: enzyme activity is affected by CG methylase (It cleaves CT5m CGAG very slowly)

Reaction temperature: 37°C

  • Source: Xanthomonas holcicola
  • Concentration: 4–12 units/µl [High concentration: 30–60 units/µl]
  • Substrate for unit definition: lambda DNA
  • Genomic DNA analysis: Escherichia coli genomic DNA
  • Ligation-recutting test: The ligation efficiency of cohesive end DNA fragments generated by this enzyme will be low. More efficient ligation can be achieved using reaction conditions for blunt end ligation
  • Each lot undergoes stringent quality testing including: overdigestion, genomic DNA analysis, ligation-recutting, and pKF3 cloning
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XhoI restriction site:

C | TCGA G
G AGCT | C

Supplied buffer: H

Effect of DNA methylation: enzyme activity is affected by CG methylase (It cleaves CT5m CGAG very slowly)

Reaction temperature: 37°C

  • Source: Xanthomonas holcicola
  • Concentration: 4–12 units/µl [High concentration: 30–60 units/µl]
  • Substrate for unit definition: lambda DNA
  • Genomic DNA analysis: Escherichia coli genomic DNA
  • Ligation-recutting test: The ligation efficiency of cohesive end DNA fragments generated by this enzyme will be low. More efficient ligation can be achieved using reaction conditions for blunt end ligation
  • Each lot undergoes stringent quality testing including: overdigestion, genomic DNA analysis, ligation-recutting, and pKF3 cloning
Write Your Own Review
You're reviewing:XhoI
Your Rating