Simple, rapid protein transfection with Xfect Protein Transfection Reagent

Simple, rapid protein transfection with Xfect Protein Transfection Reagent. Xfect’s reagent cell-penetrating activity enables proteins to be transported across membranes of mammalian cells.

How fast is direct protein transfection?

How fast is direct protein transfection? We transfected human recombinant caspase-3 using Xfect Protein Transfection Reagent and detected completion of apoptosis after just 2 hr, demonstrating that protein transfection using Xfect reagent is both fast and delivers active protein (Panel B). Other methods for inducing apoptosis require 12 hr for completion of Annexin V staining (Martin, S. J. et al. (1995) J. Exp. Med. 182(5):1545–1556.).

Xfect Protein Transfection Reagent delivers up to 8X more active beta-galactosidase into HeLa cells than the leading competitor, Product C.

Xfect Protein Transfection Reagent delivers up to 8X more active beta-galactosidase into HeLa cells than the leading competitor, Product C. Panel A. Xfect Protein Transfection Reagent or Product C was used to transfect Jurkat cells with various amount of beta-galactosidase (beta-gal) according to the manufacturers’ recommended protocol. One hour post-transfection, the cells were assayed for beta-gal activity using ourLuminescent beta-galactosidase Detection Kit II (Cat. No. 631712). Xfect Protein Transfection Reagent displayed a far higher signal for beta-gal than did Product C. Panel B. HeLa cells transfected with 4 µg of beta-gal demonstrated higher efficiency and a higher amount of beta-gal protein per cell when transfection was performed with Xfect Protein Transfection Reagent (middle panel) than with Product C (right-hand panel).

Xfect Protein Transfection Reagent delivers more recombinant fluorescent proteins than Product C into a range of cell types

Xfect Protein Transfection Reagent delivers more recombinant fluorescent proteins than Product C into a range of cell types. Xfect Protein Transfection Reagent and Product C were used to transfect a number of cell lines with purified recombinant fluorescent protein (either rAcGFP1 or rDsRed-Express). In all of the cell lines tested, a far greater MFI (mean fluorescence intensity) was detected via flow cytometry after transfection with Xfect Protein Transfection Reagent than after transfection with Product C.

Xfect Protein Transfection Reagent provides much higher cell viability than Product P when transfecting HeLa cells

Xfect Protein Transfection Reagent provides much higher cell viability than Product P when transfecting HeLa cells. HeLa cells were seeded in a 96-well plate (3 x 104 cells per well) one day prior to transfection. The cells were transfected with 0.25 µg of beta-galactosidase using Product P or Xfect Protein Transfection Reagent for 4 hours. A WST-1 proliferation assay was performed to assess cytotoxicity in the transfected samples relative to untreated HeLa cells.

Protein transfection efficiencies across different cell lines: Xfect Protein Transfection Reagent vs. the leading competitor, Product C

Protein transfection efficiencies across different cell lines: Xfect Protein Transfection Reagent vs. the leading competitor, Product C. Panel A. Xfect Protein Transfection Reagent yields higher transfection efficiencies than Product C across a broad range of mammalian cells, including a number of rodent and human cell lines, hard-to-transfect human suspension cells, and mouse embryonic stem cells. The cells were transfected with 4 µg of recombinant fluorescent protein (either rAcGFP1 or rDsRed-Express) using Xfect Protein Transfection Reagent or Product C according to the manufacturers’ recommended protocol. Transfection efficiency was assessed by flow cytometry one hour post-transfection. Panel B. HeLa transfected with 5 µg of rAcGFP1 using Product C or Xfect Protein Transfection Reagent.