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XbaI
XbaI restriction site:
T | CTAG A
A GATC | T
Supplied buffer: M+BSA
Effect of DNA methylation: enzyme activity is affected by dam methylase
Reaction temperature: 37°C
- Source: Xanthomonas badrii
- Concentration: 8–20 units/µl [High concentration: 30–60 units/µl]
- Substrate for unit definition: N6-methyladenine free lambda DNA
- Genomic DNA analysis: Escherichia coli genomic DNA
- Star activity: cleavage at alternative site(s) occurs in the presence of DMSO or high concentrations of glycerol
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
XbaI restriction site:
T | CTAG A
A GATC | T
Supplied buffer: M+BSA
Effect of DNA methylation: enzyme activity is affected by dam methylase
Reaction temperature: 37°C
- Source: Xanthomonas badrii
- Concentration: 8–20 units/µl [High concentration: 30–60 units/µl]
- Substrate for unit definition: N6-methyladenine free lambda DNA
- Genomic DNA analysis: Escherichia coli genomic DNA
- Star activity: cleavage at alternative site(s) occurs in the presence of DMSO or high concentrations of glycerol
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
