X-alpha-Gal
X-alpha-Gal
Brand: Takara Bio.
SKU: X-alpha-Gal
In stock
SKU
X-alpha-Gal
Overview
- Colonies turn blue with positive yeast two-hybrid interactions
- Direct visualization on the plate, no lysis required
X-α-Gal is a chromogenic substrate used to detect α-galactosidase activity. In yeast, α-galactosidase is a secreted reporter that is expressed from the MEL1 gene in response to GAL4 activation. α-galactosidase accumulates in the media and hydrolyses X-α-Gal causing yeast colonies to turn blue. X-α-Gal can be used with Matchmaker GAL4-based products to confirm protein interactions.
X-alpha-Gal for yeast two-hybrid studies
X-alpha-Gal is a chromogenic substrate for yeast galactosidase (MEL1) and is used for detecting GAL4-based yeast two-hybrid interactions directly on agar.
The X-alpha-Gal assay detects the activation of the yeast MEL1 gene, a GAL4-regulated gene used in yeast two-hybrid screening. MEL1 encodes the secreted enzyme alpha-galactosidase, which hydrolyzes colorless X-alpha-Gal into a blue end product. Yeast colonies that express alpha-galactosidase in response to a positive two-hybrid interaction turn blue when grown on media containing X-alpha-Gal.
X-Alpha-Gal detects secreted alpha-galactosidase activity following a GAL4-based two-hybrid interactions in Y2HGold yeast patches and colonies
X-Alpha-Gal detects secreted alpha-galactosidase activity following a GAL4-based two-hybrid interactions in Y2HGold yeast patches and colonies.
Yeast colonies that express alpha-galactosidase in response to a positive two-hybrid interaction turn blue when grown on media containing X-alpha-Gal
Yeast colonies that express alpha-galactosidase in response to a positive two-hybrid interaction turn blue when grown on media containing X-alpha-Gal.
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X-alpha-Gal for yeast two-hybrid studies
X-alpha-Gal is a chromogenic substrate for yeast galactosidase (MEL1) and is used for detecting GAL4-based yeast two-hybrid interactions directly on agar.
The X-alpha-Gal assay detects the activation of the yeast MEL1 gene, a GAL4-regulated gene used in yeast two-hybrid screening. MEL1 encodes the secreted enzyme alpha-galactosidase, which hydrolyzes colorless X-alpha-Gal into a blue end product. Yeast colonies that express alpha-galactosidase in response to a positive two-hybrid interaction turn blue when grown on media containing X-alpha-Gal.
X-Alpha-Gal detects secreted alpha-galactosidase activity following a GAL4-based two-hybrid interactions in Y2HGold yeast patches and colonies
X-Alpha-Gal detects secreted alpha-galactosidase activity following a GAL4-based two-hybrid interactions in Y2HGold yeast patches and colonies.
Yeast colonies that express alpha-galactosidase in response to a positive two-hybrid interaction turn blue when grown on media containing X-alpha-Gal
Yeast colonies that express alpha-galactosidase in response to a positive two-hybrid interaction turn blue when grown on media containing X-alpha-Gal.
