WST-1 Cell Proliferation Assay Kit
WST-1 Cell Proliferation Assay Kit
Brand: Takara Bio.
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WST-1 Cell Proliferation Assay Kit
The Premix WST-1 enables cell proliferation and cell viability to be measured with a colorimetric assay, based on cleavage of tetrazolium salts by mitochondrial dehydrogenase in viable cells. This product takes the place of RI-labeled nucleoside, and provides a non-RI method for the analysis of cell proliferation or cell viability. Washing and collecting cells is not required, and all procedures, from the culture in small scale to data analysis with a microplate reader, can be carried out in the same microtiter plate.
Overview
- Ready-to-use colorimetric assay to easily quantify cell growth and viability
- Measures cell proliferation in response to growth factors, cytokines, mitogens, & nutrients
- An effortless protocol; start to finish in one hour*
- No washing steps or additional reagents required
*Incubation time ranges from 30 min to 4 hr, depending on cell type and density
Applications
- Quantify cell proliferation and viability
Cell proliferation measurements are proportional to the concentration of the proliferation promoter IL-2
Cell proliferation measurements are proportional to the concentration of the proliferation promoter IL-2. Mouse CTLL-2 T cells were seeded in 96-well plates at 4 x 103 cells/well, with 100 µl culture medium. Increasing concentrations of human IL-2 were added, and the cells were incubated for 48 hr at 37°C with 5% CO2. 10 µl/well of Premixed WST-1 Cell Proliferation Reagent was added, and the cells were incubated for an additional 4 hr under the same conditions. Absorbance at 450 nm was measured in a multiwell plate reader.
Using the Premix WST-1 Cell Proliferation Assay to measure the cytotoxic effects of human tumor necrosis factor (TNF-alpha) on the mouse fibrosarcoma cell line WEHI-164
Using the Premix WST-1 Cell Proliferation Assay to measure the cytotoxic effects of human tumor necrosis factor (TNF-alpha) on the mouse fibrosarcoma cell line WEHI-164. WEHI-164 cells were seeded in 96-well plates at 5 x 104 cells/well in 100 μl culture medium containing increasing concentrations of TNF-alpha. The cells were incubated at 37˚C for 24 hr in a humidified atmosphere maintained at 5% CO2. 10 µl/well of Premixed WST-1 Cell Proliferation Reagent was added, and the cells were incubated for an additional 4 hr under the same conditions. Absorbance at 450 nm was measured in a multiwell plate reader.
Simple WST-1 cell-proliferation protocol
Simple WST-1 cell-proliferation protocol. Know if your cells are proliferating in under an hour.
Cleavage of the tetrazolium salt (WST-1) to formazan
Cleavage of the tetrazolium salt (WST-1) to formazan.
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Cell proliferation measurements are proportional to the concentration of the proliferation promoter IL-2
Cell proliferation measurements are proportional to the concentration of the proliferation promoter IL-2. Mouse CTLL-2 T cells were seeded in 96-well plates at 4 x 103 cells/well, with 100 µl culture medium. Increasing concentrations of human IL-2 were added, and the cells were incubated for 48 hr at 37°C with 5% CO2. 10 µl/well of Premixed WST-1 Cell Proliferation Reagent was added, and the cells were incubated for an additional 4 hr under the same conditions. Absorbance at 450 nm was measured in a multiwell plate reader.
Using the Premix WST-1 Cell Proliferation Assay to measure the cytotoxic effects of human tumor necrosis factor (TNF-alpha) on the mouse fibrosarcoma cell line WEHI-164
Using the Premix WST-1 Cell Proliferation Assay to measure the cytotoxic effects of human tumor necrosis factor (TNF-alpha) on the mouse fibrosarcoma cell line WEHI-164. WEHI-164 cells were seeded in 96-well plates at 5 x 104 cells/well in 100 μl culture medium containing increasing concentrations of TNF-alpha. The cells were incubated at 37˚C for 24 hr in a humidified atmosphere maintained at 5% CO2. 10 µl/well of Premixed WST-1 Cell Proliferation Reagent was added, and the cells were incubated for an additional 4 hr under the same conditions. Absorbance at 450 nm was measured in a multiwell plate reader.
Simple WST-1 cell-proliferation protocol
Simple WST-1 cell-proliferation protocol. Know if your cells are proliferating in under an hour.
Cleavage of the tetrazolium salt (WST-1) to formazan
Cleavage of the tetrazolium salt (WST-1) to formazan.
