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Van91I (PflMI)
Van91I (PflMI) restriction site:
CCAN NNN | NTGG
GGTN | NNN NACC
Supplied buffer: K
Effect of DNA methylation: enzyme activity is affected by dcm methylase
Reaction temperature: 37°C
- Source: Vibrio anguillarum RFL 91
- Concentration: 4–12 units/µl
- Substrate for unit definition: lambda DNA
- Star activity: unrelated site may often be cut in the presence of a high concentration of glycerol
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
Van91I (PflMI) restriction site:
CCAN NNN | NTGG
GGTN | NNN NACC
Supplied buffer: K
Effect of DNA methylation: enzyme activity is affected by dcm methylase
Reaction temperature: 37°C
- Source: Vibrio anguillarum RFL 91
- Concentration: 4–12 units/µl
- Substrate for unit definition: lambda DNA
- Star activity: unrelated site may often be cut in the presence of a high concentration of glycerol
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
