Universal Primer Mix
Universal Primer Mix
Brand: Takara Bio.
In stock
SKU
Universal Primer Mix
The Universal Primer Mix (UPM) adds suppression PCR elements to the ends of cDNA generated by our SMARTer RACE 5'/3' Kit (Cat. Nos. 634858 & 634859). The UPM consists of two primers: a long, 45-base primer and a short, 22-base primer. The Long Universal Primer (Long UP) is composed of two parts: the 23 bases at the 3' end of the primer are identical to those at the 5' end of the SMARTer IIA Oligo; the 22 bases at the 5' end of the primer provide the suppression sequence for suppression PCR. The Short Universal Primer (Short UP) sequence is identical to that of the 22 bases at the 5' end of the Long UP. The Short UP, for which the binding site is introduced by the Long UP during the first round of PCR, is present in the UPM at 5 times the concentration of the Long UP.
Applications
- RACE (Rapid Amplification of cDNA Ends)
- Identification of 3'/5' UTRs
- Full-length gene cloning
- Gene cloning and analysis
Bertling, W. M., Beier, F. & Reichenberger, E. Determination of 5' ends of specific mRNAs by DNA ligase-dependent amplification. PCR Methods Appl. 3, 95–9 (1993).
Frohman, M. A. Rapid amplification of complementary DNA ends for generation of full-length complementary DNAs: thermal RACE. Methods Enzymol. 218, 340–56 (1993).
Write Your Own Review
