Transgene Detection Primer Set (Mouse)
Transgene Detection Primer Set (Mouse)
Brand: Takara Bio.
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Transgene Detection Primer Set (Mouse)
Transgene detection primer set
This kit is a primer set for real-time PCR to detect GFP (EGFP, AcGFP1) and lacZ, which are gene markers in transgenic mice. It is possible to screen transgenic mice by detecting these marker genes with real-time PCR. The primer sets (Ywhaz, Raver2) for detecting genes on mouse genomic DNA are also included as a reference. Therefore, it is possible to make a comparison of the transgene contents between samples by relative quantification.
Applications
- Detection and quantification of mouse genome transgenes
Components
Sufficient for 100 reactions with each primer when using TB Green Premix Ex Taq II with 25 µl reaction volumes.
Notes:
1GFP primer-1 is designed for detection of EGFP and AcGFP1 sequences.
2GFP primer-2 is designed for detection of EGFP sequence. It cannot be used for AcGFP1 detection.
3lacZ primer-1 and -2 are designed for lacZ (beta-galactosidase) gene detection and target different gene sequences.
4Reference primer-1 is designed for a part of the Ywhaz gene on mouse chromosome 15.
5Reference primer-2 is designed for a part of the Raver2 gene on mouse chromosome 4.
Determination of homozygous/heterozygous genotypes using the Transgene Detection Primer Set for Real Time (Mouse) This experiment demonstrates how the Transgene Detection Primer Set for Real Time (Mouse) and quantitative PCR of genomic DNA from mouse tails can be used to accurately determine the homozygous and heterozygous genotypes of transgenic mice
Determination of homozygous/heterozygous genotypes using the Transgene Detection Primer Set for Real Time (Mouse)
This experiment demonstrates how the Transgene Detection Primer Set for Real Time (Mouse) and quantitative PCR of genomic DNA from mouse tails can be used to accurately determine the homozygous and heterozygous genotypes of transgenic mice.
Five different mice representing three different genotypes for the EGFP and lacZ transgenes were used for the experiment.
Tg#1 and Tg#3 were homozygous for the transgenes
Tg#2 and Tg#4 were heterozygous
wild-type (Wt) mouse had neither transgene*
*The mouse tails were kindly provided by Drs. Ikenaka and Takebayashi, National Institutes of Natural Sciences, Japan.
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Components
Sufficient for 100 reactions with each primer when using TB Green Premix Ex Taq II with 25 µl reaction volumes.
Notes:
1GFP primer-1 is designed for detection of EGFP and AcGFP1 sequences.
2GFP primer-2 is designed for detection of EGFP sequence. It cannot be used for AcGFP1 detection.
3lacZ primer-1 and -2 are designed for lacZ (beta-galactosidase) gene detection and target different gene sequences.
4Reference primer-1 is designed for a part of the Ywhaz gene on mouse chromosome 15.
5Reference primer-2 is designed for a part of the Raver2 gene on mouse chromosome 4.
Determination of homozygous/heterozygous genotypes using the Transgene Detection Primer Set for Real Time (Mouse) This experiment demonstrates how the Transgene Detection Primer Set for Real Time (Mouse) and quantitative PCR of genomic DNA from mouse tails can be used to accurately determine the homozygous and heterozygous genotypes of transgenic mice
Determination of homozygous/heterozygous genotypes using the Transgene Detection Primer Set for Real Time (Mouse)
This experiment demonstrates how the Transgene Detection Primer Set for Real Time (Mouse) and quantitative PCR of genomic DNA from mouse tails can be used to accurately determine the homozygous and heterozygous genotypes of transgenic mice.
Five different mice representing three different genotypes for the EGFP and lacZ transgenes were used for the experiment.
Tg#1 and Tg#3 were homozygous for the transgenes
Tg#2 and Tg#4 were heterozygous
wild-type (Wt) mouse had neither transgene*
*The mouse tails were kindly provided by Drs. Ikenaka and Takebayashi, National Institutes of Natural Sciences, Japan.
