Titanium Taq SP DNA Polymerase

Titanium Taq SP DNA Polymerase

Brand: Takara Bio.
In stock
SKU
Titanium Taq SP DNA Polymerase
Grouped product items
Product Name Size
Titanium® Taq SP DNA Polymerase
SKU: 639292
100 Rxns
Make An Enquiry
× Product Enquiry
Titanium Taq SP DNA Polymerase
Titanium Taq SP DNA Polymerase

Fill in the details below:

Titanium Taq SP—high-yield DNA polymerase for laboratory testing (GPR-registered)

Titanium Taq SP DNA Polymerase is a registered general purpose reagent (GPR) appropriate for use in general laboratory applications, including molecular diagnostic development and testing. Titanium Taq SP contains the same Titanium Taq DNA Polymerase and TaqStart Antibody as our standard Titanium Taq products, but with a modified, license-free buffer formulation. Titanium Taq SP performs similarly to our standard Titanium Taq in all tested customer applications. It is ideal for PCR amplification of most DNA templates, and for complex genomic DNA in particular.

Titanium Taq is more robust than wild-type Taq polymerases and allows the amplification of highly complex DNA mixes due to the absence of 5'-exonuclease activity found in wild-type Taq. This novel enzyme contains carefully engineered amino acid substitutions that increase its solubility, making it incredibly sensitive.

Overview

  • Manufactured and labeled in accordance with requirements for general purpose reagents
  • Functionally tested to help ensure remarkable lot-to-lot reproducibility, consistency, and a wide dynamic range
  • Amplify your target using fewer PCR cycles, thus reducing background
  • Perform PCR without optimizing your reaction conditions—Titanium Taq tolerates a wide range of Mg2+ concentrations
  • Amplify targets of up to 2 kb from highly complex templates, such as mammalian genomic DNA
  • Amplifies rare or low-copy targets
  • Set up your PCR at room temperature and see increased specificity and yield due to the integrated hot-start antibody

Applications

  • Genotyping
  • Whole-genome PCR
  • Multiplex PCR
  • Primer extension
  • Hot-start PCR
  • Preparative PCR
  • Rare-template amplification

Titanium Taq efficiently amplifies specific genes from genomic DNA

Titanium Taq efficiently amplifies specific genes from genomic DNA

Titanium Taq efficiently amplifies specific genes from genomic DNA. Human cardiac beta-myosin heavy chain fragments of different lengths were amplified from 100 ng of genomic DNA using Titanium Taq and two leading competitor’s hot start Taq polymerases. Optimal conditions were used for each enzyme, as specified by the manufacturer. Lane 1: 0.5 kb fragment. Lane 2: 1 kb fragment. Lane 3: 1.8 kb fragment. Lane M: 1 kb DNA size ladder.

TITANIUM Taq is active over a wide range of Mg2+ concentrations

TITANIUM Taq is active over a wide range of Mg2+ concentrations

TITANIUM Taq is active over a wide range of Mg2+ concentrations. TITANIUM was used to amplify a 500 bp region of Calf Thymus genomic DNA. The MgCl2 concentration was varied as indicated. The enzyme performed consistently through the range of Mg2+ used. Lane M: DNA size marker.

Write Your Own Review
You're reviewing:Titanium Taq SP DNA Polymerase
Your Rating

Titanium Taq SP—high-yield DNA polymerase for laboratory testing (GPR-registered)

Titanium Taq SP DNA Polymerase is a registered general purpose reagent (GPR) appropriate for use in general laboratory applications, including molecular diagnostic development and testing. Titanium Taq SP contains the same Titanium Taq DNA Polymerase and TaqStart Antibody as our standard Titanium Taq products, but with a modified, license-free buffer formulation. Titanium Taq SP performs similarly to our standard Titanium Taq in all tested customer applications. It is ideal for PCR amplification of most DNA templates, and for complex genomic DNA in particular.

Titanium Taq is more robust than wild-type Taq polymerases and allows the amplification of highly complex DNA mixes due to the absence of 5'-exonuclease activity found in wild-type Taq. This novel enzyme contains carefully engineered amino acid substitutions that increase its solubility, making it incredibly sensitive.

Overview

  • Manufactured and labeled in accordance with requirements for general purpose reagents
  • Functionally tested to help ensure remarkable lot-to-lot reproducibility, consistency, and a wide dynamic range
  • Amplify your target using fewer PCR cycles, thus reducing background
  • Perform PCR without optimizing your reaction conditions—Titanium Taq tolerates a wide range of Mg2+ concentrations
  • Amplify targets of up to 2 kb from highly complex templates, such as mammalian genomic DNA
  • Amplifies rare or low-copy targets
  • Set up your PCR at room temperature and see increased specificity and yield due to the integrated hot-start antibody

Applications

  • Genotyping
  • Whole-genome PCR
  • Multiplex PCR
  • Primer extension
  • Hot-start PCR
  • Preparative PCR
  • Rare-template amplification

Titanium Taq efficiently amplifies specific genes from genomic DNA

Titanium Taq efficiently amplifies specific genes from genomic DNA

Titanium Taq efficiently amplifies specific genes from genomic DNA. Human cardiac beta-myosin heavy chain fragments of different lengths were amplified from 100 ng of genomic DNA using Titanium Taq and two leading competitor’s hot start Taq polymerases. Optimal conditions were used for each enzyme, as specified by the manufacturer. Lane 1: 0.5 kb fragment. Lane 2: 1 kb fragment. Lane 3: 1.8 kb fragment. Lane M: 1 kb DNA size ladder.

TITANIUM Taq is active over a wide range of Mg2+ concentrations

TITANIUM Taq is active over a wide range of Mg2+ concentrations

TITANIUM Taq is active over a wide range of Mg2+ concentrations. TITANIUM was used to amplify a 500 bp region of Calf Thymus genomic DNA. The MgCl2 concentration was varied as indicated. The enzyme performed consistently through the range of Mg2+ used. Lane M: DNA size marker.

Write Your Own Review
You're reviewing:Titanium Taq SP DNA Polymerase
Your Rating