Titanium Taq SP—high-yield DNA polymerase for laboratory testing (GPR-registered)
Titanium Taq SP DNA Polymerase is a registered general purpose reagent (GPR) appropriate for use in general laboratory applications, including molecular diagnostic development and testing. Titanium Taq SP contains the same Titanium Taq DNA Polymerase and TaqStart Antibody as our standard Titanium Taq products, but with a modified, license-free buffer formulation. Titanium Taq SP performs similarly to our standard Titanium Taq in all tested customer applications. It is ideal for PCR amplification of most DNA templates, and for complex genomic DNA in particular.
Titanium Taq is more robust than wild-type Taq polymerases and allows the amplification of highly complex DNA mixes due to the absence of 5'-exonuclease activity found in wild-type Taq. This novel enzyme contains carefully engineered amino acid substitutions that increase its solubility, making it incredibly sensitive.
Overview
Manufactured and labeled in accordance with requirements for general purpose reagents
Functionally tested to help ensure remarkable lot-to-lot reproducibility, consistency, and a wide dynamic range
Amplify your target using fewer PCR cycles, thus reducing background
Perform PCR without optimizing your reaction conditions—Titanium Taq tolerates a wide range of Mg2+ concentrations
Amplify targets of up to 2 kb from highly complex templates, such as mammalian genomic DNA
Amplifies rare or low-copy targets
Set up your PCR at room temperature and see increased specificity and yield due to the integrated hot-start antibody
Titanium Taq efficiently amplifies specific genes from genomic DNA
Titanium Taq efficiently amplifies specific genes from genomic DNA. Human cardiac beta-myosin heavy chain fragments of different lengths were amplified from 100 ng of genomic DNA using Titanium Taq and two leading competitor’s hot start Taq polymerases. Optimal conditions were used for each enzyme, as specified by the manufacturer. Lane 1: 0.5 kb fragment. Lane 2: 1 kb fragment. Lane 3: 1.8 kb fragment. Lane M: 1 kb DNA size ladder.
TITANIUM Taq is active over a wide range of Mg2+ concentrations
TITANIUM Taq is active over a wide range of Mg2+ concentrations. TITANIUM was used to amplify a 500 bp region of Calf Thymus genomic DNA. The MgCl2 concentration was varied as indicated. The enzyme performed consistently through the range of Mg2+ used. Lane M: DNA size marker.
Titanium Taq SP—high-yield DNA polymerase for laboratory testing (GPR-registered)
Titanium Taq SP DNA Polymerase is a registered general purpose reagent (GPR) appropriate for use in general laboratory applications, including molecular diagnostic development and testing. Titanium Taq SP contains the same Titanium Taq DNA Polymerase and TaqStart Antibody as our standard Titanium Taq products, but with a modified, license-free buffer formulation. Titanium Taq SP performs similarly to our standard Titanium Taq in all tested customer applications. It is ideal for PCR amplification of most DNA templates, and for complex genomic DNA in particular.
Titanium Taq is more robust than wild-type Taq polymerases and allows the amplification of highly complex DNA mixes due to the absence of 5'-exonuclease activity found in wild-type Taq. This novel enzyme contains carefully engineered amino acid substitutions that increase its solubility, making it incredibly sensitive.
Overview
Manufactured and labeled in accordance with requirements for general purpose reagents
Functionally tested to help ensure remarkable lot-to-lot reproducibility, consistency, and a wide dynamic range
Amplify your target using fewer PCR cycles, thus reducing background
Perform PCR without optimizing your reaction conditions—Titanium Taq tolerates a wide range of Mg2+ concentrations
Amplify targets of up to 2 kb from highly complex templates, such as mammalian genomic DNA
Amplifies rare or low-copy targets
Set up your PCR at room temperature and see increased specificity and yield due to the integrated hot-start antibody
Titanium Taq efficiently amplifies specific genes from genomic DNA
Titanium Taq efficiently amplifies specific genes from genomic DNA. Human cardiac beta-myosin heavy chain fragments of different lengths were amplified from 100 ng of genomic DNA using Titanium Taq and two leading competitor’s hot start Taq polymerases. Optimal conditions were used for each enzyme, as specified by the manufacturer. Lane 1: 0.5 kb fragment. Lane 2: 1 kb fragment. Lane 3: 1.8 kb fragment. Lane M: 1 kb DNA size ladder.
TITANIUM Taq is active over a wide range of Mg2+ concentrations
TITANIUM Taq is active over a wide range of Mg2+ concentrations. TITANIUM was used to amplify a 500 bp region of Calf Thymus genomic DNA. The MgCl2 concentration was varied as indicated. The enzyme performed consistently through the range of Mg2+ used. Lane M: DNA size marker.