Titanium One-Step RT-PCR Kit
Titanium One-Step RT-PCR Kit
Brand: Takara Bio.
In stock
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Titanium One-Step RT-PCR Kit
The Titanium One-Step RT-PCR Kit is designed for the reverse transcription of total or poly A+ RNA and the subsequent PCR amplification of a target cDNA in a single reaction tube. The included enzyme mix contains a highly purified Moloney murine leukemia virus (MMLV) reverse transcriptase for first-strand cDNA synthesis, and Titanium Taq DNA Polymerase for PCR amplification. Titanium Taq DNA Polymerase is a blend of a 5'-exonuclease deficient, thermostable DNA polymerase, and TaqStart Antibody for automatic hot start PCR. Reagents for purifying RNA are not supplied.
Overview
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Allows qualitative analysis of gene expression from multiple samples
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Produces results from as little as 10 pg of total RNA
- Provided in a convenient single-tube format
- Easy-to-use one-step format minimizes the risk of cross contamination
Applications
- One-step RT-PCR
- Endpoint RT-PCR
- Gene expression analysis
- First-strand cDNA synthesis
Titanium One-Step RT-PCR is more efficient than other commercially available one-step kits
Titanium One-Step RT-PCR is more efficient than other commercially available one-step kits. Reactions were performed in parallel using the Titanium One-Step RT-PCR Kit and two other commercially available one-step kits under the manufacturers' recommended conditions. RT-PCR products were analyzed via agarose/EtBr gel electrophoresis. Human placenta total RNA (1 μg) was used as template for each reaction, and nine different human transcripts were amplified. Lane M: DNA size marker.
Superior sensitivity of Titanium One-Step RT-PCR
Superior sensitivity of Titanium One-Step RT-PCR. Different amounts of mouse liver total RNA were used as template for TITANIUM One-Step RT-PCR to amplify the beta-actin transcript. The target transcript was reverse-transcribed at 50°C for 1 hr and amplified using 40 PCR cycles; RT-PCR products were analyzed via EtBr/agarose gel electrophoresis. Lane 1: 1 μg of total RNA. Lane 2: 100 ng. Lane 3: 10 ng. Lane 4: 1 ng. Lane 5: 100 pg. Lane 6: 10 pg. Lane 7: 1 pg. Lane 8: no template. Lane M: DNA size markers.
References
Gerard, G. F., et al., Reverse transcriptase (EC 2.7.7.49): the use of cloned maloney murine leukemia virus reverse transcriptase to synthesize DNA from RNA. In Burrell,M.M. (ed.). Methods in Molecular Biology: Enzymes of Molecular Biology. (Humana Press, Inc., Totowa, NJ), 16:73–93 (1993).
Kellogg, D. E., et al., TaqStart Antibody: "hot start" PCR facilitated by a neutralizing monoclonal antibody directed against Taq DNA polymerase. BioTechniques 16:1134–1137 (1994).
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Titanium One-Step RT-PCR is more efficient than other commercially available one-step kits
Titanium One-Step RT-PCR is more efficient than other commercially available one-step kits. Reactions were performed in parallel using the Titanium One-Step RT-PCR Kit and two other commercially available one-step kits under the manufacturers' recommended conditions. RT-PCR products were analyzed via agarose/EtBr gel electrophoresis. Human placenta total RNA (1 μg) was used as template for each reaction, and nine different human transcripts were amplified. Lane M: DNA size marker.
Superior sensitivity of Titanium One-Step RT-PCR
Superior sensitivity of Titanium One-Step RT-PCR. Different amounts of mouse liver total RNA were used as template for TITANIUM One-Step RT-PCR to amplify the beta-actin transcript. The target transcript was reverse-transcribed at 50°C for 1 hr and amplified using 40 PCR cycles; RT-PCR products were analyzed via EtBr/agarose gel electrophoresis. Lane 1: 1 μg of total RNA. Lane 2: 100 ng. Lane 3: 10 ng. Lane 4: 1 ng. Lane 5: 100 pg. Lane 6: 10 pg. Lane 7: 1 pg. Lane 8: no template. Lane M: DNA size markers.
