Tet-On 3G Transactivator Lentiviral Particles
Tet-On 3G Transactivator Lentiviral Particles
Brand: Takara Bio.
In stock
SKU
Tet-On 3G Transactivator Lentiviral Particles
Highly concentrated and purified lentivirus for delivery of the Tet-On 3G transactivator to target cells. Any gene of interest under the control of a TRE3G promoter can be tightly controlled by the presence and absence of Dox in the culture medium. Tet-On 3G is a modified, improved Tet-On Advanced transcriptional activator protein (transactivator) which has been evolved to display far higher sensitivity to the inducer molecule dox. Tet-On 3G generates very high maximal expression and responds to lower Dox concentrations than its predecessors.
Overview
- What does 109 TU/ml mean?
- In each microliter, there will be at least 1 x 106 functional lentivirus particles.
- Each 60-µl kit is sufficient for 30 transductions (using an MOI of 10) in a 6-well plate containing 2 x 105 cells/well.
- Gold standard tetracycline transactivator in a ready-to-use lentivirus format.
- Premium quality; produced via a multistep purification process which results in very high titers and purity.
- Functional titer provided, not a just a particle count.
- High purity—transduce target cells without cytotoxicity.
- >90% transduction efficiency can be obtained even at very low MOI.
- Can be combined with any vector containing a TRE promoter to obtain tetracycline-inducible expression of your gene of interest (we recommend the TRE3G promoter).
- Learn more about Tet-On 3G technology.
More Information
Applications
- Tightly controlled, inducible expression of your gene of interest in hard-to-transfect mammalian cells
Various cell types can be transduced with Vectalys lentiviral particles, including primary and stem cell lines
Various cell types can be transduced with Vectalys lentiviral particles, including primary and stem cell lines. The suggested seeding concentration and MOI vary depending on the specific cell type to be transduced. When the recommendations in this table are followed, transduction efficiency is high for immortalized, primary, and stem cells, regardless of whether they are adherent or suspension cells.
Overview of the process for generating high-titer lentiviral particles
Overview of the process for generating high-titer lentiviral particles. Through this proprietary, large-scale process Vectalys is able to produce very high-titer, high-purity lentiviruses ready for transduction of many different cell types. The high titer allows for manipulation of even hard-to-transduce cells, like primary cells or stem cells, and the exceptional purity prevents cytotoxicity.
Transduction efficiency of a Vectalys GFP-expressing lentiviral vector in IMR90 cells
Transduction efficiency of a Vectalys GFP-expressing lentiviral vector in IMR90 cells. Even with a low MOI of 5, >95% of cells are transduced using this high-titer lentivirus. Very high expression can be achieved by increasing the MOI.
Comparison of the purity of lentiviruses generated with Vectalys’ method or a classic purification method
Comparison of the purity of lentiviruses generated with Vectalys’ method or a classic purification method. Lentiviral proteins were separated by SDS-PAGE and stained with Coomassie Blue. The high purity of Vectalys lentiviral particles is clear when comparing the two methods; even when Vectalys particles are loaded at a 10-fold higher amount, very little contaminating protein is seen. The ratio of total particles (physical particles) to functional particles (transducing units) is lower for Vectalys purified lentivirus compared to classic methods of purification, indicating higher purity. Data provided by Vectalys.
Not all titers are the same! Comparison of cells transduced with 100 M
Not all titers are the same! Comparison of cells transduced with 100 M.O.I. of lentivirus provided by Clontech/Vectalys versus 100 M.O.I. of lentivirus provided by Vendor S. Each premade virus preparation expresses a green fluorescent protein when transduced. Clontech/Vectalys provides a functional titer, so every cell treated with our virus preparation was transduced with multiple copies of virus, leading to strong expression of the fluorescent protein. By contrast, the titer provided by Vendor S includes nonfunctional lentivirus particles as a large percentage of the titer so 100 M.O.I of their virus preparation is not sufficient to effectively transduce the cells.
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Various cell types can be transduced with Vectalys lentiviral particles, including primary and stem cell lines
Various cell types can be transduced with Vectalys lentiviral particles, including primary and stem cell lines. The suggested seeding concentration and MOI vary depending on the specific cell type to be transduced. When the recommendations in this table are followed, transduction efficiency is high for immortalized, primary, and stem cells, regardless of whether they are adherent or suspension cells.
Overview of the process for generating high-titer lentiviral particles
Overview of the process for generating high-titer lentiviral particles. Through this proprietary, large-scale process Vectalys is able to produce very high-titer, high-purity lentiviruses ready for transduction of many different cell types. The high titer allows for manipulation of even hard-to-transduce cells, like primary cells or stem cells, and the exceptional purity prevents cytotoxicity.
Transduction efficiency of a Vectalys GFP-expressing lentiviral vector in IMR90 cells
Transduction efficiency of a Vectalys GFP-expressing lentiviral vector in IMR90 cells. Even with a low MOI of 5, >95% of cells are transduced using this high-titer lentivirus. Very high expression can be achieved by increasing the MOI.
Comparison of the purity of lentiviruses generated with Vectalys’ method or a classic purification method
Comparison of the purity of lentiviruses generated with Vectalys’ method or a classic purification method. Lentiviral proteins were separated by SDS-PAGE and stained with Coomassie Blue. The high purity of Vectalys lentiviral particles is clear when comparing the two methods; even when Vectalys particles are loaded at a 10-fold higher amount, very little contaminating protein is seen. The ratio of total particles (physical particles) to functional particles (transducing units) is lower for Vectalys purified lentivirus compared to classic methods of purification, indicating higher purity. Data provided by Vectalys.
Not all titers are the same! Comparison of cells transduced with 100 M
Not all titers are the same! Comparison of cells transduced with 100 M.O.I. of lentivirus provided by Clontech/Vectalys versus 100 M.O.I. of lentivirus provided by Vendor S. Each premade virus preparation expresses a green fluorescent protein when transduced. Clontech/Vectalys provides a functional titer, so every cell treated with our virus preparation was transduced with multiple copies of virus, leading to strong expression of the fluorescent protein. By contrast, the titer provided by Vendor S includes nonfunctional lentivirus particles as a large percentage of the titer so 100 M.O.I of their virus preparation is not sufficient to effectively transduce the cells.
