Terra PCR Direct Genotyping Kit
Terra PCR Direct Genotyping Kit
Brand: Takara Bio.
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Terra PCR Direct Genotyping Kit
Terra PCR Direct Genotyping Kit
This Genotyping Kit contains everything needed to genotype directly from animal tissue such as mouse tails. The Kit is based on the hot-start Terra PCR Direct DNA Polymerase Mix, a highly sensitive enzyme that allows amplification of targets from small amounts of template. In addition, the enzyme readily amplifies short DNA targets (up to 2 kb), regardless of GC content or template purity. Tissue Extract Buffer and PCR buffer (with Mg2+ and dNTPs) are included in the kit. A tube of Proteinase K is provided to enhance gel detection of the PCR products.
Overview
- Skip sample prep
- Amplify directly from animal or plant tissues, or from crude extracts
- Easily amplify GC-rich targets
- Room-temperature setup with automatic hot start
- Specialized kits including a convenient premix, an all-in-one kit that includes extraction buffer and loading dye, and FFPE and Whatman FTA sample processing formulations
Applications
- Genotyping
- Direct PCR from crude extracts, tissues, blood, FFPE samples and many more
Terra PCR Direct was used to amplify the cyclin D2 gene (Ccnd2, 0.5 kb; Lane 1) and the transferrin receptor gene (TfrC 2 kb; Lane 2) from 1 μl of mouse blood treated with either EDTA or heparin
Panel A. Terra PCR Direct was used to amplify the cyclin D2 gene (Ccnd2, 0.5 kb; Lane 1) and the transferrin receptor gene (TfrC, 2 kb; Lane 2) from 1 μl of mouse blood treated with either EDTA or heparin. Panel B. Terra PCR Direct was used to amplify the mouse Ywhaz1 gene (1 kb) directly from either a 1 mm tail or 1.5 mm2 ear biopsy. A 4 μl aliquot of each sample was mixed with gel loading buffer that either lacked or contained proteinase K (Lanes 1 and 2, respectively). The PCR products treated with proteinase K ran as expected, whereas those without proteinase K treatment got stuck in the wells. Panel C. Terra PCR Direct was used to amplify the cytochrome c oxidase gene (cox1; 0.5 kb) directly from 0.5 mm (Lane 1) and 1.2 mm (Lane 2) tomato or spinach leaf cuttings (made using hole punches).
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Terra PCR Direct was used to amplify the cyclin D2 gene (Ccnd2, 0.5 kb; Lane 1) and the transferrin receptor gene (TfrC 2 kb; Lane 2) from 1 μl of mouse blood treated with either EDTA or heparin
Panel A. Terra PCR Direct was used to amplify the cyclin D2 gene (Ccnd2, 0.5 kb; Lane 1) and the transferrin receptor gene (TfrC, 2 kb; Lane 2) from 1 μl of mouse blood treated with either EDTA or heparin. Panel B. Terra PCR Direct was used to amplify the mouse Ywhaz1 gene (1 kb) directly from either a 1 mm tail or 1.5 mm2 ear biopsy. A 4 μl aliquot of each sample was mixed with gel loading buffer that either lacked or contained proteinase K (Lanes 1 and 2, respectively). The PCR products treated with proteinase K ran as expected, whereas those without proteinase K treatment got stuck in the wells. Panel C. Terra PCR Direct was used to amplify the cytochrome c oxidase gene (cox1; 0.5 kb) directly from 0.5 mm (Lane 1) and 1.2 mm (Lane 2) tomato or spinach leaf cuttings (made using hole punches).
