Terminal Deoxynucleotidyl Transferase
Terminal Deoxynucleotidyl Transferase
Brand: Takara Bio.
In stock
SKU
Terminal Deoxynucleotidyl Transferase
Terminal Deoxynucleotidyl Transferase (TdT)
Terminal Deoxynucleotidyl Transferase (TdT) catalyzes the incorporation of single deoxynucleotides into the 3'-OH terminus of single- or double-stranded DNA, making it an effective agent in 3' end labeling applications such as Terminal dUTP nick end labeling (TUNEL). Terminal Deoxynucleotidyl Transferase (TdT) requires an oligodeoxynucleotide of at least three bases as a primer, but is not template-dependent.
Applications
Okayama-Berg methods involving complementary homopolymer tailing of vectors and cDNA
3'-end labeling of DNA Fragments using [32P]dNTP or [32P]ddNTP
TUNEL
Supplied buffer
- 5X TdT Buffer [500 mM HEPES (pH 7.2), 40 mM MgCl2, 0.5 mM DTT]
- 0.1% BSA
Purity
Endonuclease, exonuclease and nickase activities were not detected via gel electrophoresis.
Form
60 mM potassium phosphate (pH 7.2), 150 mM KCl, 1 mM 2-mercaptoethanol, 50% glycerol
Write Your Own Review
