TB Green Premix Ex Taq (Tli RNase H Plus)

TB Green Premix Ex Taq (Tli RNase H Plus) provides reduced PCR inhibition caused by the presence of double-stranded RNA/cDNA hybrids remaining after cDNA synthesis. This inhibition is common when using low or RNase H minus RTs, and with GC-rich templates and/or genes with poor expression. The presence of dsRNA/cDNA hybrids is a frequent cause of poor qPCR amplification and/or reaction failure. TB Green Premix Ex Taq (Tli RNase H Plus) prevents this potential problem at no additional cost and without requiring a separate RNase H digestion step prior to qPCR.

Overview

Lower Cq values as a result of fast extension rates
Use with broad range of amplicon sizes, even over 500 bp
Convenient 2X master mix is excellent for high-speed qPCR
High sensitivity: detects fewer than 100 copies
Hot-start PCR enzyme enables high efficiency and sensitivity in real-time PCR (qPCR)
Compatible with all commonly used real-time PCR (qPCR) instruments
Minimizes qPCR inhibition due to residual mRNA in input cDNA
Includes TB Green for intercalator-based qPCR

Applications

TB Green qPCR using TB Green and ROX Reference Dye
Gene expression analysis
Genotyping by real-time PCR
High-throughput real-time PCR
Amplification of long products

Product citations

Kim, et al. Characterization of naturally Epstein-Barr virus-infected gastric carcinoma cell line YCCEL1. J. Gen. Virol. 94, 497–506 (2013).

Kotani, et al. Systemic Circulation and Bone Recruitment of Osteoclast Precursors Tracked by Using Fluorescent Imaging Techniques. J. Immunol. 190, 605–612 (2013).

Liu, et al. Metabolism and Pharmacokinetics of Mangiferin in Conventional Rats, Pseduo-Germ-Free Rats, and Streptozotocin-Induced Diabetic Rats. Drug Metab. Dispos. 40, 2109–2118 (2012).