TB Green Premix Ex Taq II (Tli RNase H Plus)

TB Green Premix Ex Taq II (Tli RNase H Plus) is a reagent specifically designed for intercalator-based real time PCR using TB Green. It is supplied at a 2X concentration premixed with TB Green at a concentration appropriate for real time monitoring, making it easy to prepare reaction mixtures. The 2X premixed reagent also contains Tli RNase H, a heat-resistant RNase H, which minimizes PCR inhibition due to residual mRNA when using cDNA as template. This product, with a modified buffer composition, offers a higher reaction specificity than that of TB Green Premix Ex Taq (Tli RNaseH Plus) (Cat.# RR420A). With inhibition of non-specific amplifications, which interfere with quantitative determination, accurate assays over a wide range are possible. A combination of this buffer and Takara Ex Taq HS, a hot start PCR enzyme that uses an anti-Taq antibody, allows highly reproducible and reliable real time PCR analyses.

Overview

High specificity minimizes primer-dimer formation and nonspecific amplification
Excels with GC-rich targets
2X master mix provides convenience
High sensitivity: detects fewer than 100 copies
Compatible with all commonly used qPCR instruments
Includes TB Green Master Mix for intercalator-based qPCR
Tli RNase H Plus reduces inhibition from mRNA/cDNA hybrids in qPCR
Eliminates the need for a separate RNase H digestion step when using low RNase H RT

Applications

Gene expression analysis
qPCR
Genotyping
Suppression of primer dimers or off-target products

Components

Two ROX reference dyes are supplied as separate components with Cat. # RR820A, RR820B, RR820L, and RR820W. These reference dyes serve as convenient internal reference standards when normalizing non-PCR generated fluorescence fluctuations; such fluctuations often occur between different wells or over time in different instruments.
Cat. # RR820L and RR820W are larger package sizes (5 ml and 25 ml (5 x 5 ml), respectively) of TB Green Premix Ex Taq II (Tli RNase H Plus). Each of these includes a separate vial of ROX Reference Dye.
Cat. # RR82LR and RR82WR are larger package sizes (5 ml and 25 ml (5 x 5 ml), respectively) with ROX Reference Dye included in the premix.

Product citations

Hong, S. et al. Type II protein arginine methyltransferase 5 (PRMT5) is required for circadian period determination in Arabidopsis thaliana. Proc. Natl. Acad. Sci. USA 107:21211–21216 (2010).

Ishikawa, M. et al. Downregulation of glutamine synthetase via GLAST suppression induces retinal axonal swelling in a rat ex vivo hydrostatic pressure model. Invest. Ophthalmol. Vis. Sci. 52:6604–6616 (2011).