A monoclonal antibody to Taq DNA polymerase for use in hot-start PCR. By binding to Taq polymerase, this antibody inhibits the polymerase activity and prevent non-specific amplificatioon derived from mispriming and/or formation of primer dimers before PCR cycling. The antibody is denatured during the initial denaturation step, so there is no need for a special denaturation step. Taq polymerase can be used in general PCR conditions.
Following incubation of Taq antibody and Taq polymerase at 25°C for 10 min, inhibition of Taq polymerase activity was confirmed as greater than 90% (at 55°C for 10 min).
Following heat treatment at 94°C for 30 seconds, Taq antibody-bound Taq polymerase was confirmed to recover almost 100% activity (at 55°C for 10 minutes).
A monoclonal antibody to Taq DNA polymerase for use in hot-start PCR. By binding to Taq polymerase, this antibody inhibits the polymerase activity and prevent non-specific amplificatioon derived from mispriming and/or formation of primer dimers before PCR cycling. The antibody is denatured during the initial denaturation step, so there is no need for a special denaturation step. Taq polymerase can be used in general PCR conditions.
Following incubation of Taq antibody and Taq polymerase at 25°C for 10 min, inhibition of Taq polymerase activity was confirmed as greater than 90% (at 55°C for 10 min).
Following heat treatment at 94°C for 30 seconds, Taq antibody-bound Taq polymerase was confirmed to recover almost 100% activity (at 55°C for 10 minutes).
