TaKaRa Taq DNA Polymerase and premix

TaKaRa Taq DNA Polymerase is a recombinant version Taq polymerase derived from the Thermus aquaticus YT-1 strain, and is suitable for routine PCR applications.

For individual reaction setup and optimization, use individual components of enzyme, dNTP mixture, and 10X reaction buffer (with or without Mg2+). A convenient 2X PCR master mix of Takara Taq enzyme, buffer, and dNTP mixture is available as Premix Taq DNA Polymerase (TaKaRa Taq Version 2.0) (Cat. # R004A).

Two other formulations of TaKaRa Taq DNA polymerase are also available:

Several antibody-mediated hot-start versions of Takara Taq—for preventing non-specific amplification from mispriming during reaction setup
GMP-grade version—for an added level of confidence in our quality polymerase

Overview

Excellent for standard PCR amplifications
Low bacterial DNA contamination
Premix Taq enables easy PCR setup and minimizes pipetting steps

Applications

Routine PCR
DNA sequencing

Source

Recombinant E. coli

PCR products

The resulting PCR product can be cloned directly into a T-vector (TA cloning) due to the addition of an adenosine (A) to the 3' end. It is also possible to clone the PCR product into blunt-end vectors after blunting and phosphorylation of the ends.

Amplification of various fragments from lambda DNA (1 ng) using Takara Taq DNA Polymerase

Amplification of various fragments from lambda DNA (1 ng) using Takara Taq DNA Polymerase. The PCR products were analyzed by agarose gel electrophoresis (lane 1, 4 kb; lane 2, 6 kb; lane 3, 8 kb; lane 4, 10 kb; lane M, lambda Hind III DNA Marker).

Amplification of a single-copy gene (TP53) from human placental genomic DNA (100 ng) template using Takara Taq DNA Polymerase

Amplification of a single-copy gene (TP53) from human placental genomic DNA (100 ng) template using Takara Taq DNA Polymerase. The amplification products were resolved by gel electrophoresis (lane 1, 1.2 kb; lane 2, 2.9 kb; lane M, lambda-Hind III DNA Marker).

Quality control results for Takara Taq DNA Polymerase and a Taq enzyme from Company A

Quality control results for Takara Taq DNA Polymerase and a Taq enzyme from Company A. Because Taq DNA polymerase is typically expressed in E. coli, it is especially important to test the polymerase for the presence of contaminating E. coli genomic DNA. Quality control testing of Takara Taq polymerase for contamination was performed by nested PCR of the E. coli genomic DNA Ori region. The results demonstrate that Takara Taq enzyme is a low-DNA-contamination-grade enzyme.

Properties and Characteristics of Takara Taq DNA Polymerase

Properties and Characteristics of Takara Taq DNA Polymerase.