DNA Ligation Kit, Long Fragments
DNA Ligation Kit, Long Fragments
Brand: Takara Bio.
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DNA Ligation Kit, Long Fragments
DNA ligation kit for long fragments
Our DNA ligation kit for long fragments is a powerful tool for cloning long DNA fragments spanning 2 kb to over 10 kb in length. This kit contains an optimized ligase/buffer system that enables ligation of long fragments without the need for extensive techniques or special expertise. It is especially well-suited for BAC library construction. Compared with other systems, the TaKaRa DNA Ligation Kit LONG results in insertion efficiencies up to 50X higher for high-molecular-weight DNA fragments ranging from 2 kb to 18 kb than other kits. In addition, this kit also enables fast and easy ligation of smaller-sized DNA inserts.
Overview
- Superior ligation efficiency, especially for long DNA fragments
- Simple protocol
- Long control vector and insert provided
Applications
- Vector construction using long DNA fragments
- BAC library construction
- Cloning of long cDNA fragments
Time course of cohesive-end ligation: 8 kb DNA fragments digested with Hind III were Ligated into the pUC 8/Hind III/BAP vector using the protocol described in the user manual
Time course of cohesive-end ligation: 8 kb DNA fragments digested with Hind III were Ligated into the pUC 8/Hind III/BAP vector using the protocol described in the user manual. Ligation products were transformed into chemically competent cells and grown overnight on LB-amp plates incubated at 37°C.
Comparison of ligation efficiency with various DNA ligation sites
Comparison of ligation efficiency with various DNA ligation sites. DNA fragments (2 kb, 4 kb, 10 kb and 18 kb) digested with Hind III were ligated into the cloning vector pUC118/Hind III/BAP using the Takara Bio Ligation Kit Long (Lig LONG) or other commerically available DNA ligation kits. Ligation products were transformed into E. coli DH5alpha cells and grown overnight on LB-amp plates at 37 degrees C.
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Time course of cohesive-end ligation: 8 kb DNA fragments digested with Hind III were Ligated into the pUC 8/Hind III/BAP vector using the protocol described in the user manual
Time course of cohesive-end ligation: 8 kb DNA fragments digested with Hind III were Ligated into the pUC 8/Hind III/BAP vector using the protocol described in the user manual. Ligation products were transformed into chemically competent cells and grown overnight on LB-amp plates incubated at 37°C.
Comparison of ligation efficiency with various DNA ligation sites
Comparison of ligation efficiency with various DNA ligation sites. DNA fragments (2 kb, 4 kb, 10 kb and 18 kb) digested with Hind III were ligated into the cloning vector pUC118/Hind III/BAP using the Takara Bio Ligation Kit Long (Lig LONG) or other commerically available DNA ligation kits. Ligation products were transformed into E. coli DH5alpha cells and grown overnight on LB-amp plates at 37 degrees C.
