T4 RNA Ligase is a ligation enzyme for joining single-stranded RNA or DNA fragments by catalyzing the formation of phosphodiester bonds between single-stranded RNA or DNA containing 5'-phosphate and 3'-hydroxyl termini. This enzyme requires ATP for activity, and its activity is highest for RNA-RNA ligation, lower for RNA-DNA ligation, and lowest for DNA-DNA ligation. 3'-end labeling is often completed using 5'-[32P]pCp.
One unit is defined as the amount of enzyme that converts 1 pmol of [5'-32P]pCp into its acid-insoluble form in 10 minutes at 5°C, using oligo(A) as the substrate during 3' end labeling of RNA.
T4 RNA Ligase is a ligation enzyme for joining single-stranded RNA or DNA fragments by catalyzing the formation of phosphodiester bonds between single-stranded RNA or DNA containing 5'-phosphate and 3'-hydroxyl termini. This enzyme requires ATP for activity, and its activity is highest for RNA-RNA ligation, lower for RNA-DNA ligation, and lowest for DNA-DNA ligation. 3'-end labeling is often completed using 5'-[32P]pCp.
One unit is defined as the amount of enzyme that converts 1 pmol of [5'-32P]pCp into its acid-insoluble form in 10 minutes at 5°C, using oligo(A) as the substrate during 3' end labeling of RNA.
