SuperPlex Premix
SuperPlex Premix
Brand: Takara Bio.
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SuperPlex Premix
SuperPlex Premix PCR master mix designed for fast, uniform multiplexing
SuperPlex Premix is a 2X, hot-start PCR master mix formulated with our high-yield Titanium Taq DNA polymerase. SuperPlex Premix is ideal for multiplex PCR where unbiased amplification of different targets and high amplicon yield are important. The premix formulation minimizes pipetting steps and streamlines workflows.
Overview
- Formulated to minimize the time required to optimize multiplex reactions
- High-yield enzyme requires fewer PCR cycles and generates low background signal
- Convenient premix formulation simplifies your workflow, saving time and reducing potential errors
Applications of multiplex PCR
- Pathogen detection/identification
- High-throughput SNP genotyping
- Mutation analysis
- Gene deletion analysis
- Template quantification
Successful amplification of 20-plex PCR with a short PCR cycle time using SuperPlex Premix, but not other multiplex master mixes
Successful amplification of 20-plex PCR with a short PCR cycle time using SuperPlex Premix, but not other multiplex master mixes. We compared the performance of SuperPlex Premix to four other multiplex PCR master mixes using the recommended SuperPlex Premix PCR cycle. Each lane represents the PCR products from a 20-plex reaction obtained from a different premix, visualized with ethidium bromide staining. Lane 1: SuperPlex Premix. Lane 2: KAPA2G Fast Multiplex Mix. Lane 3: Platinum Multiplex PCR Master Mix. Lane 4: NEB Multiplex PCR 5X Master Mix. Lane 5: Qiagen Multiplex PCR Kit, without Q-solution. Lane 6: Qiagen Multiplex PCR Kit, with Q-solution.
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Successful amplification of 20-plex PCR with a short PCR cycle time using SuperPlex Premix, but not other multiplex master mixes
Successful amplification of 20-plex PCR with a short PCR cycle time using SuperPlex Premix, but not other multiplex master mixes. We compared the performance of SuperPlex Premix to four other multiplex PCR master mixes using the recommended SuperPlex Premix PCR cycle. Each lane represents the PCR products from a 20-plex reaction obtained from a different premix, visualized with ethidium bromide staining. Lane 1: SuperPlex Premix. Lane 2: KAPA2G Fast Multiplex Mix. Lane 3: Platinum Multiplex PCR Master Mix. Lane 4: NEB Multiplex PCR 5X Master Mix. Lane 5: Qiagen Multiplex PCR Kit, without Q-solution. Lane 6: Qiagen Multiplex PCR Kit, with Q-solution.
