Stellar Electrocompetent Cells
Stellar Electrocompetent Cells
Brand: Takara Bio.
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Stellar Electrocompetent Cells
Stellar Electrocompetent Cells for cloning
Our Stellar Electrocompetent Cells provide high transformation efficiency, making them suitable for many molecular biology applications, such as generation of cDNA libraries or constructing gene banks. This E. coli strain can be used in place of DH10B cells and has several useful features. The recA1 and endA1 markers in the strain minimize recombination and degradation of plasmid DNA. These electrocompetent cells, based on the HST08 E. coli strain, provide alpha-complementation of the beta-galactosidase gene for blue/white screening of recombinant colonies (positive clones).
Stellar Electrocompetent Cells lack the gene cluster which digests foreign methylated DNA (mrr-hsdRMS-mcrBC and mcrA) and are therefore useful for cloning of methylated DNA, in addition to the construction of standard and longer-length genomic libraries.
Applications
- Cloning of large fragments
- cDNA library construction
- Genomic library construction
- Long-length genomic library construction
Transformation efficiency
>1 × 109 cfu/µg
Genotype
F-, endA1, supE44, thi-1, recA1, relA1, gyrA96, phoA, Φ80d lacZΔ M15, Δ (lacZYA - argF) U169, Δ (mrr - hsdRMS - mcrBC), ΔmcrA, λ-
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