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SphI
SphI restriction site:
G CATG | C
C | GTAC G
Supplied buffer: H
Effect of DNA methylation: enzyme activity is not affected by CG methylase
Reaction temperature: 37°C
- Source: Streptomyces phaeochromogenes
- Concentration: 4–12 units/µl
- Substrate for unit definition: lambda DNA
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
SphI restriction site:
G CATG | C
C | GTAC G
Supplied buffer: H
Effect of DNA methylation: enzyme activity is not affected by CG methylase
Reaction temperature: 37°C
- Source: Streptomyces phaeochromogenes
- Concentration: 4–12 units/µl
- Substrate for unit definition: lambda DNA
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
