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SpeI
SpeI restriction site:
A | CTAG T
T GATC | A
Supplied buffer: M
Reaction temperature: 37°C
- Source: Sphaerotilus natans
- Concentration: 4–12 units/µl
- Substrate for unit definition: pASf2-XbaI DNA
- Genome DNA analysis: Escherichia coli genomic DNA
- Star activity: cleavage at alternative site(s) occurs in the presence of DMSO or at low ionic strength
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
SpeI restriction site:
A | CTAG T
T GATC | A
Supplied buffer: M
Reaction temperature: 37°C
- Source: Sphaerotilus natans
- Concentration: 4–12 units/µl
- Substrate for unit definition: pASf2-XbaI DNA
- Genome DNA analysis: Escherichia coli genomic DNA
- Star activity: cleavage at alternative site(s) occurs in the presence of DMSO or at low ionic strength
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
