SpeedSTAR HS DNA Polymerase
SpeedSTAR HS DNA Polymerase
Brand: Takara Bio.
In stock
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SpeedSTAR HS DNA Polymerase
SpeedSTAR HS DNA Polymerase
SpeedSTAR HS DNA Polymerase is optimized for hot-start, fast PCR, with possible extension time as fast as 10 sec/kb (compared to 60 sec/kb with general enzymes), and amplicon size up to 20 kb. SpeedSTAR reactions can be performed using standard PCR instrumentation.
Overview
- Optimized for fast PCR: amplify a 2-kb fragment in as little as 30 minutes using a standard PCR machine
- Excellent efficiency: robust performance, comparable to high-yield polymerases
- Optimized buffers allow amplification of fragments up to 20 kb
Applications
- Fast PCR: reactions can be completed in two-thirds less time
- Amplification of large DNA fragments
PCR products
The majority of PCR products generated with SpeedSTAR DNA Polymerase will have 3'-A overhangs; therefore, amplification products can be directly cloned into T-vectors. It is also possible to clone amplification products into blunt-ended vectors following blunting and phosphorylation of product ends.
Amplification of an 8.5 kb Human Genomic DNA Fragment using a Standard High Yield Polymerase and SpeedSTAR HS DNA Polymerase
Amplification of an 8.5 kb Human Genomic DNA Fragment using a Standard High Yield Polymerase and SpeedSTAR HS DNA Polymerase.
Amplification of Human Genomic DNA Targets of Varying Sizes using SpeedSTAR High speed amplification is especially valuable when amplifying large-size targets
Amplification of Human Genomic DNA Targets of Varying Sizes using SpeedSTAR High speed amplification is especially valuable when amplifying large-size targets. This data shows excellent specificity and yield.
Time Comparison of SpeedSTAR and Standard High Efficiency Enzyme Reaction Times on E. coli Fragments of Varying Sizes (2-step refers to PCR cycling conditions)
Time Comparison of SpeedSTAR and Standard High Efficiency Enzyme Reaction Times on E. coli Fragments of Varying Sizes (2-step refers to PCR cycling conditions).
Time Comparison of SpeedSTAR and Standard High Efficiency Enzyme Reaction Times on Large Size Human Genomic Targets (2-step refers to PCR cycler conditions)
Time Comparison of SpeedSTAR and Standard High Efficiency Enzyme Reaction Times on Large Size Human Genomic Targets (2-step refers to PCR cycler conditions).
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Amplification of an 8.5 kb Human Genomic DNA Fragment using a Standard High Yield Polymerase and SpeedSTAR HS DNA Polymerase
Amplification of an 8.5 kb Human Genomic DNA Fragment using a Standard High Yield Polymerase and SpeedSTAR HS DNA Polymerase.
Amplification of Human Genomic DNA Targets of Varying Sizes using SpeedSTAR High speed amplification is especially valuable when amplifying large-size targets
Amplification of Human Genomic DNA Targets of Varying Sizes using SpeedSTAR High speed amplification is especially valuable when amplifying large-size targets. This data shows excellent specificity and yield.
Time Comparison of SpeedSTAR and Standard High Efficiency Enzyme Reaction Times on E. coli Fragments of Varying Sizes (2-step refers to PCR cycling conditions)
Time Comparison of SpeedSTAR and Standard High Efficiency Enzyme Reaction Times on E. coli Fragments of Varying Sizes (2-step refers to PCR cycling conditions).
Time Comparison of SpeedSTAR and Standard High Efficiency Enzyme Reaction Times on Large Size Human Genomic Targets (2-step refers to PCR cycler conditions)
Time Comparison of SpeedSTAR and Standard High Efficiency Enzyme Reaction Times on Large Size Human Genomic Targets (2-step refers to PCR cycler conditions).
