ThruPLEX Plasma-Seq Kit
ThruPLEX Plasma-Seq Kit
Brand: Takara Bio.
In stock
SKU
ThruPLEX Plasma-Seq Kit
The SMARTer ThruPLEX Plasma-Seq Kit builds on the innovative ThruPLEX chemistry to generate high-complexity DNA libraries from cell-free DNA isolated from plasma. Single index, dual index, and unique dual index kits are available and must be purchased separately.
Features
- Designed for cell-free DNA—newly formulated repair and ligation reagents with optimized protocols
- High-performance NGS libraries—high library diversity with broad and reproducible GC coverage
- Variable sample input—<1 ng to 30 ng of cell-free DNA
- Fast and simple workflow—three steps in a single tube or well in two hours, with no purification or sample transfer steps (tubes and plates sold separately)
- Research applications—construct libraries from samples where results require high sensitivity, including liquid biopsies, ctDNA, and targeted sequencing
- Automation friendly—compatible with the Beckman FXP Workstation
SMARTer DNA HT Dual Index kits
SMARTer DNA HT Dual Index kits are designed for use with SMARTer ThruPLEX and SMARTer PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the Illumina Nextera® XT v2 index sequences and offer a total of 384 dual indexes for multiplexing of up to 384 samples. The indexed PCR primers are supplied pre-dispensed in four different barcoded index plates, each of which contains one-fourth of the possible dual index combinations (Cat. # R400660–R400663); or in a set of 24 individual tubes, each containing a different dual index combination (Cat. # R400664). Each dual index tube (24N) contains sufficient volume for up to two uses. Each well of a dual index plate (96N Sets A–D) contains sufficient volume for a single use.
SMARTer DNA Unique Dual Index kits
SMARTer DNA Unique Dual Index kits are designed for use with SMARTer ThruPLEX and SMARTer PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the "IDT for Illumina UD" index sequences and offer a total of 96 dual indexes for multiplexing of up to 96 samples. The indexed PCR primers are supplied pre-dispensed in four different sets of 24 individual tubes, each of which contains one-fourth of the possible dual index combinations (Cat. Nos. R400665–R400668). Each dual index tube (24U Sets A–D) contains sufficient volume for up to two uses.
SMARTer DNA Single Index kits
SMARTer DNA Single Index kits are designed for use with SMARTer ThruPLEX and SMARTer PicoPLEX library preparation kits to construct libraries for multiplexed sequencing on Illumina sequencers. These kits contain indexed PCR primers carrying the "TruSeq LT set A" index sequences and offer a total of 12 single indexes for multiplexing of up to 12 samples. The indexed PCR primers are supplied pre-dispensed in four different sets of 12 individual tubes, each containing a different index sequence (Cat. Nos. R400695 and R400697). Each single index tube (12S Sets A–B) contains sufficient volume for up to eight uses.
Guelfi, G. et al. Next generation sequencing of urine exfoliated cells: an approach of prostate cancer microRNAs research. Sci. Rep. 8, 7111 (2018).
Mayrhofer, M. et al. Cell-free DNA profiling of metastatic prostate cancer reveals microsatellite instability, structural rearrangements and clonal hematopoiesis. Genome Med. 10, 85–98 (2018).
Mouliere, F. et al. Detection of cell‐free DNA fragmentation and copy number alterations in cerebrospinal fluid from glioma patients. EMBO 12, e9323 (2018).
Murtaza, M. et al. Non-invasive analysis of acquired resistance to cancer therapy by sequencing of plasma DNA. Nature 497, 108–112 (2013).
Patel, K. M. et al. Association of plasma and urinary mutant DNA with clinical outcomes in muscle invasive bladder cancer. Sci. Rep. 7, 5554 (2017).
Xia, Y. et al. Copy number variations in urine cell free DNA as biomarkers in advanced prostate cancer. Oncotarget 7, 35818–35831 (2016).
Write Your Own Review
