Strand-specific Illumina sequencing libraries from picogram RNA inputs of any quality

The SMARTer Stranded Total RNA-Seq Kit v3 - Pico Input Mammalian is designed for efficient preparation of Illumina sequencing libraries from picogram input amounts (250 pg–10 ng) of high-quality, partially degraded, or low-quality total RNA, including total RNA prepared from FFPE or LCM samples. Updates to our original kit include improved sequencing performance on all Illumina platforms, as well as seamless integration of unique molecular identifiers (UMIs) into the library preparation workflow.

The kit features a streamlined workflow that retains strand information and incorporates indexes and adapters during the reverse-transcription and PCR-amplification steps. The entire protocol, including library prep and purification, takes only about 7.5 hours.

This kit comes prepackaged with the SMARTer RNA Unique Dual Index Kit - 24U (Cat. # 634451) and can also be used with our other SMARTer RNA Unique Dual Index Kits (Cat. # 634452 & 634457; sold separately) for larger reaction sizes, allowing library construction with up to 192 different combinations of Illumina indexes.

Please note that we will continue to provide and support the original SMARTer Stranded Total RNA-Seq Kit - Pico Input Mammalian and the SMARTer Stranded Total RNA-Seq Kit v2 - Pico Input Mammalian for your convenience.

Overview

• Accommodates picogram inputs of starting material—start from 250 pg–10 ng of total RNA or 10–1,000 intact cells from human, mouse, or rat
• Seamless integration of UMIs—accurately identify true variants and rare mutations and mitigate potential PCR amplification bias and sequencing errors
• Versatile—generate reproducible data from RNA of any quality (including FFPE and LCM samples and cell-free RNA)
• Fast, streamlined protocol—go from start to finish in ~7.5 hours and save time during library purification with an improved buffer formulation
• Compatible with Illumina sequencing—generate Illumina-ready libraries with up to 192 combinations of indexes

Applications

• Robust NGS library construction that retains strand information
• RNA-seq on all Illumina platforms
• Coding and noncoding information from total mammalian RNA of any quality, including RNA obtained from FFPE samples