SMART cDNA Library Construction Kit
SMART cDNA Library Construction Kit
Brand: Takara Bio.
In stock
SKU
SMART cDNA Library Construction Kit
A kit for making seven cDNA libraries from nanogram amounts of total or poly A+ RNA. The kit utilizes the proprietary SMART (Switching Mechanism At 5' end of RNA Template) Oligonucleotide to generate high yields of full-length ds cDNA. Control Poly A+ RNA, Control Insert and pre-digested λTriplEx2 are included.
Overview
SMART cDNA Library Construction Kit (Cat. # 634901)
- Generate cDNA from as little as 50 ng of total RNA
- Enrich for full-length cDNA
- Every cDNA inserted into the MCS is expressed in three reading frames
- Utilizes rare SfiI sites to ensure your cDNA is intact
- No adapter ligation is required for cDNA synthesis or cloning
Applications
- Large-insert cDNA library construction
- Full-length cDNA amplification
- Directional cDNA cloning, adapter-free
- Sequencing
SMART(er) cDNA synthesis compared to conventional cDNA synthesis
SMART(er) cDNA synthesis compared to conventional cDNA synthesis. Unlike conventional cDNA synthesis methods, which involve a multiple enzyme/multiple step procedure, the SMART(er) cDNA synthesis protocol is performed by one reverse transcription reaction, in a single tube, with no adaptor ligation or intervening purification steps. Following PCR amplification, SMART(er) cDNA is immediately available for a variety of downstream applications.
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SMART(er) cDNA synthesis compared to conventional cDNA synthesis
SMART(er) cDNA synthesis compared to conventional cDNA synthesis. Unlike conventional cDNA synthesis methods, which involve a multiple enzyme/multiple step procedure, the SMART(er) cDNA synthesis protocol is performed by one reverse transcription reaction, in a single tube, with no adaptor ligation or intervening purification steps. Following PCR amplification, SMART(er) cDNA is immediately available for a variety of downstream applications.
