Sialic Acid Fluorescence Labeling Kit
Sialic Acid Fluorescence Labeling Kit
Brand: Takara Bio.
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Sialic Acid Kit
The fluorescence labeling of sialic acid with 1, 2-diamino-4, 5-methyleneoxybenzene (DMB) is a simple and very sensitive analytical method in which free sialic acids are analyzed by reverse-phase HPLC after labeling with DMB. The sialic acids which are bound to the sugar chains in glycoproteins or glycolipids can also be measured after pre-treatment with sialidases or acid hydrolysis. The detection limit of DMB-sialic acid is 57 fmol. TaKaRa's Sialic Acid Fluorescence Labeling Kit is designed to simplify the DMB-derivatization procedure. The reagents are provided in aqueous solutions and the protocol only involves mixing of reagents and incubation. By combination of this DMB method with Pyridylamino (PA) derivatization, sialoglycoconjugates can be analyzed with extremely high sensitivity.
Applications
- Quantitative and highly sensitive sialoglycoconjugate analysis
- Sialic acid HPLC
Hara, S. et al., Determination of mono-O-acetylated N-acetylneuraminic acids in human and rat sera by fluorometric high performance liquid chromatography. Anal. Biochem. 179,162–166 (1989).
Klein, A. et al., New sialic acids from biological sources identified by a comprehensive and sensitive approach: liquid chromatography-electrospray ionization-mass spectrometry (LC-ESI-MS) of SIA quinoxalinones. Glycobiology 7, 421–432 (1997).
Nakamura, M. et al., 1, 2-Diamino-4, 5-methylenedioxybenzene as a Highly Sensitive Fluorogenic Reagent for α-Keto Acids. Chem. Pharm. Bull. 35, 687–692 (1987).
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