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SfiI
SfiI restriction site:
GGCCN NNN | NGGCC
CCGGN | NNN NCCGG
Supplied buffer: M
Effect of DNA methylation: enzyme activity is often affected by dcm methylase
Reaction temperature: 50°C
- Source: Streptomyces fimbriatus
- Concentration: 4–12 units/µl
- Substrate for unit definition: Ad2 DNA
- Genome DNA analysis: Escherichia coli genomic DNA
- Star activity: cleavage at alternative site(s) occurs in the presence of Mn2+ or DMSO
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
SfiI restriction site:
GGCCN NNN | NGGCC
CCGGN | NNN NCCGG
Supplied buffer: M
Effect of DNA methylation: enzyme activity is often affected by dcm methylase
Reaction temperature: 50°C
- Source: Streptomyces fimbriatus
- Concentration: 4–12 units/µl
- Substrate for unit definition: Ad2 DNA
- Genome DNA analysis: Escherichia coli genomic DNA
- Star activity: cleavage at alternative site(s) occurs in the presence of Mn2+ or DMSO
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
