SapphireAmp fast PCR—hot-start master mix

SapphireAmp Fast PCR Master Mix is designed for fast, streamlined PCR, and is, therefore, ideal for applications such as colony PCR. It contains a hot-start PCR enzyme, optimized buffer, dNTPs, along with a blue gel-loading dye and density reagent, in a convenient 2X premix format.

Reactions are assembled by simply mixing primers and DNA template with SapphireAmp Fast PCR Master Mix. For rapid and convenient screening, the amplification reactions can be loaded directly onto an agarose gel immediately after amplification without purification, or digested directly with restriction enzymes.

For E. coli-based colony PCR, inserts up to ~5 kb in length are easily screened; colony PCR can be performed in about an hour.

For human genomic DNA, amplification of 2-kb targets can be completed in one hour; amplifications of up to 6 kb are possible.

Overview

• Hot-start PCR master mix is optimized for fast PCR
• Colony PCR can be completed in half the time required for conventional Taq DNA polymerase
• Convenient protocol eliminates purification steps with no reduction in yield
• Restriction enzyme digestion of the PCR products can be performed directly in the PCR buffer
• Reactions can be directly loaded onto a gel without purification or the addition of other reagents

Applications

• Hot-start PCR
• Colony PCR
• Fast PCR