SAN High Quality (Bioprocessing grade) is the ultimate solution for efficient removal of nucleic acids in manufacturing and bioprocessing workflows. This nonspecific, recombinant endonuclease has optimum activity at high salt concentrations, which can improve efficiency and yield in various workflows.
The Main Advantages of SAN High Quality
High purity (≥ 98%)
No protease detected
Animal origin-free production
Endotoxin-tested
Active at low temperatures
High activity at high salt conditions
Supplied with extended product documentation
Compatible with SAN HQ ELISA
Properties
Source: Recombinantly produced in Pichia pastoris
Specific activity: ≥ 175 000 Units/mg
Size: The protein is glycosylated. Protein size without glycosylation is 26 kDa
Unit definition: One Unit is defined as an increase in absorbance at 260 nm of 1 A in 30 minutes at 37°C, using 50 μg/ml calf thymus DNA in a buffer consisting of 25 mM Tris-HCl, pH 8.5 (25°C), 5 mM MgCl2, 500 mM NaCl
Specificity: Nonspecific endonuclease cleaving single- and double stranded DNA and RNA. The enzyme degrades DNA vs. RNA in a 10:1 ratio
End-product: Following complete degradation, the end product consist of a majority of 5 nucleotide oligos
Activity:
Tmp: 0–40°C, optimal 35°C
NaCl: 50mM–1M, optimal NaCl is 0.5 M
Mg2+ (>1 mM) is required for activity, optimal Mg2+ is 20 mM
pH: 7.0–9.5, optimal pH is 9
Tolerance to typical buffer additives:
Imidazole: 20% activity at 350 mM Imidazole
Glycerol: 20% activity at 35% glycerol
Triton X-100: No reduction in activity (tested up to 15%)
SDS: Not recommended
Urea: Not recommended
Reducing agents (e.g. DTT, TCEP): will result in inactivation
SAN HQ ELISA Kit
ArcticZymes offers SAN HQ ELISA Kit to confirm the removal of SAN High Quality (Bioprocessing Grade) in bioprocessing and biomanufacturing applications.
SAN High Quality (Bioprocessing grade) is the ultimate solution for efficient removal of nucleic acids in manufacturing and bioprocessing workflows. This nonspecific, recombinant endonuclease has optimum activity at high salt concentrations, which can improve efficiency and yield in various workflows.
Salt is an important component of various purification schemes. The presence of salt can minimize aggregation, increase target solubility and improve target yield. High salt enables contaminating DNA to dissociate from associated proteins and become available for degradation. SAN High Quality is highly compatible with the use of high salt conditions.
SAN High Quality (Bioprocessing grade) is the ultimate solution for efficient removal of nucleic acids in manufacturing and bioprocessing workflows. This nonspecific, recombinant endonuclease has optimum activity at high salt concentrations, which can improve efficiency and yield in various workflows.
The Main Advantages of SAN High Quality
High purity (≥ 98%)
No protease detected
Animal origin-free production
Endotoxin-tested
Active at low temperatures
High activity at high salt conditions
Supplied with extended product documentation
Compatible with SAN HQ ELISA
Properties
Source: Recombinantly produced in Pichia pastoris
Specific activity: ≥ 175 000 Units/mg
Size: The protein is glycosylated. Protein size without glycosylation is 26 kDa
Unit definition: One Unit is defined as an increase in absorbance at 260 nm of 1 A in 30 minutes at 37°C, using 50 μg/ml calf thymus DNA in a buffer consisting of 25 mM Tris-HCl, pH 8.5 (25°C), 5 mM MgCl2, 500 mM NaCl
Specificity: Nonspecific endonuclease cleaving single- and double stranded DNA and RNA. The enzyme degrades DNA vs. RNA in a 10:1 ratio
End-product: Following complete degradation, the end product consist of a majority of 5 nucleotide oligos
Activity:
Tmp: 0–40°C, optimal 35°C
NaCl: 50mM–1M, optimal NaCl is 0.5 M
Mg2+ (>1 mM) is required for activity, optimal Mg2+ is 20 mM
pH: 7.0–9.5, optimal pH is 9
Tolerance to typical buffer additives:
Imidazole: 20% activity at 350 mM Imidazole
Glycerol: 20% activity at 35% glycerol
Triton X-100: No reduction in activity (tested up to 15%)
SDS: Not recommended
Urea: Not recommended
Reducing agents (e.g. DTT, TCEP): will result in inactivation
SAN HQ ELISA Kit
ArcticZymes offers SAN HQ ELISA Kit to confirm the removal of SAN High Quality (Bioprocessing Grade) in bioprocessing and biomanufacturing applications.
SAN High Quality (Bioprocessing grade) is the ultimate solution for efficient removal of nucleic acids in manufacturing and bioprocessing workflows. This nonspecific, recombinant endonuclease has optimum activity at high salt concentrations, which can improve efficiency and yield in various workflows.
Salt is an important component of various purification schemes. The presence of salt can minimize aggregation, increase target solubility and improve target yield. High salt enables contaminating DNA to dissociate from associated proteins and become available for degradation. SAN High Quality is highly compatible with the use of high salt conditions.