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SalI
SalI restriction site:
G | TCGA C
C AGCT | G
Supplied buffer: H
Effect of DNA methylation: enzyme activity is affected by CG methylase
Reaction temperature: 37°C
- Source: Streptomyces albus G
- Concentration: 8–20 units/µl [High concentration: 30–60 units/µl]
- Substrate for unit definition: lambda DNA
- Genomic DNA analysis: Staphylococcus aureus genomic DNA
- Star activity: cleavage at alternative site(s) occurs in the presence of DMSO or high concentrations of glycerol
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
SalI restriction site:
G | TCGA C
C AGCT | G
Supplied buffer: H
Effect of DNA methylation: enzyme activity is affected by CG methylase
Reaction temperature: 37°C
- Source: Streptomyces albus G
- Concentration: 8–20 units/µl [High concentration: 30–60 units/µl]
- Substrate for unit definition: lambda DNA
- Genomic DNA analysis: Staphylococcus aureus genomic DNA
- Star activity: cleavage at alternative site(s) occurs in the presence of DMSO or high concentrations of glycerol
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
