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SacII
SacII restriction site:
CC GC | GG
GG | CG CC
Supplied buffer: T+BSA
Effect of DNA methylation: enzyme activity is affected by CG methylase
Reaction temperature: 37°C
- Source: Streptomyces achromogenes
- Concentration: 8–20 units/µl
- Substrate for unit definition: Ad2 DNA
- Genome DNA analysis: Staphylococcus aureus genomic DNA
- This enzyme is able to cleave at 4 lambda DNA sites, but the site at position 40386 is resistant to cleavage
- Each lot undergoes stringent quality testing, including: overdigestion, genome DNA analysis, ligation-recutting, and pKF3 Cloning
SacII restriction site:
CC GC | GG
GG | CG CC
Supplied buffer: T+BSA
Effect of DNA methylation: enzyme activity is affected by CG methylase
Reaction temperature: 37°C
- Source: Streptomyces achromogenes
- Concentration: 8–20 units/µl
- Substrate for unit definition: Ad2 DNA
- Genome DNA analysis: Staphylococcus aureus genomic DNA
- This enzyme is able to cleave at 4 lambda DNA sites, but the site at position 40386 is resistant to cleavage
- Each lot undergoes stringent quality testing, including: overdigestion, genome DNA analysis, ligation-recutting, and pKF3 Cloning
