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SacI
SacI restriction site:
G AGCT | C
C | TCGA G
Supplied buffer: L
Effect of DNA methylation: enzyme activity is not affected by CG methylase
Reaction temperature: 37°C
- Source: Streptomyces achromogenes
- Concentration: 4–12 units/µl [High concentration: 30–60 units/µl]
- Substrate for unit definition: lambda DNA
- Star activity: cleavage at alternative site(s) occurs in the presence of DMSO or high concentrations of glycerol
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
SacI restriction site:
G AGCT | C
C | TCGA G
Supplied buffer: L
Effect of DNA methylation: enzyme activity is not affected by CG methylase
Reaction temperature: 37°C
- Source: Streptomyces achromogenes
- Concentration: 4–12 units/µl [High concentration: 30–60 units/µl]
- Substrate for unit definition: lambda DNA
- Star activity: cleavage at alternative site(s) occurs in the presence of DMSO or high concentrations of glycerol
- Each lot undergoes stringent quality testing including overdigestion, genome DNA analysis, ligation-recutting and pKF3 cloning
