RNA LA PCR Kit
The RNA LA PCR Kit is designed to generate longer and more accurate RT-PCR products. The kit uses AMV RT XL as the reverse transcriptase, which allows for efficient synthesis of first-strand cDNA up to 12 kb. AMV RT XL also provides better thermostability than other reverse transcriptases, and retains its activity over a wider range of reaction temperatures. In addition, the kit uses LA Taq, which can synthesize products up to 40 kb in size with 6.5X better fidelity than Taq Polymerase. Both the reverse transcription and amplification reactions can be performed in a single tube. The components for 3'-RACE are also supplied.
Overview
- Enables first-strand cDNA synthesis from templates up to 12 kb in length
Applications
- Two-step RT-PCR using AMV RT XL and reverse transcriptase
- Amplification of longer cDNA templates following reverse transcription using Takara LA Taq
Amplification of long mRNA (approx. 12 kb) using the RNA LA PCR Kit
Amplification of long mRNA (approx. 12 kb) using the RNA LA PCR Kit. The RNA LA PCR Kit was used to amplify several long fragments from the dystrophin gene (6, 8, and 12 kb) from human heart mRNA. This data shows excellent performance, even on very long products. Template: Human heart mRNA. Target cDNA: Dystrophin (6, 8, 12 kb).
Schematic diagram of RNA PCR using the RNA LA PCR Kit, Version 1.1
Schematic diagram of RNA PCR using the RNA LA PCR Kit, Version 1.1.
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Amplification of long mRNA (approx. 12 kb) using the RNA LA PCR Kit
Amplification of long mRNA (approx. 12 kb) using the RNA LA PCR Kit. The RNA LA PCR Kit was used to amplify several long fragments from the dystrophin gene (6, 8, and 12 kb) from human heart mRNA. This data shows excellent performance, even on very long products. Template: Human heart mRNA. Target cDNA: Dystrophin (6, 8, 12 kb).
Schematic diagram of RNA PCR using the RNA LA PCR Kit, Version 1.1
Schematic diagram of RNA PCR using the RNA LA PCR Kit, Version 1.1.
