RHB-A Neural Stem Cell Culture Medium
RHB-A Neural Stem Cell Culture Medium
Brand: Takara Bio.
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RHB-A Neural Stem Cell Culture Medium
RHB-A, supplemented with Epidermal Growth Factor (EGF) and Fibroblast Growth Factor-2 (FGF-2), enables the maintenance and continual expansion of symmetrically-dividing NS cells in defined, serum-free adherent culture. In growth factor supplemented RHB-A, NS cells have been demonstrated to retain their neurogenic capacity for over 100 generations, with full maintenance of diploid karyotype. RHB-A in the presence of EGF and FGF-2 also supports the derivation of clonogenic NS cell lines. Culture of adherent NS cells in RHB-A with sequential growth factor withdrawal leads to differentiation into functional neurons. RHB-A supplemented with EGF and FGF-2 has recently been used for the propagation of glioblastoma stem cell lines.
RHB-Basal is a proprietary, defined, serum-free basal medium specifically formulated for the propagation and differentiation of adherent NS cells. RHB-Basal does not contain any growth factors or neuronal supplements. Therefore, customers can tailor RHB-Basal to suit the specific requirements of their cell type by the addition of preferred supplements.
Overview
- RHB-A is a proprietary, fully defined, and serum-free medium designed to maintain pure populations of adherent human and mouse NS cells
- RHB-Basal medium is free of animal components and neuronal supplements; the medium can be customized by the addition of supplements
Applications
- Derivation of mouse and human NS cells from ES cells and fetal and adult tissues
- Maintenance and propagation of adherent mouse and human NS cells
- Differentiation of mouse and human NS cells into functional neurons
- Differentiation of mouse ES cells to neuronal precursors
- Organotypic slice culture of CNS tissue
- Glioblastoma stem cell culture for cancer research
- Refer to the Data Sheet for additional examples of use
Adherent human neural stem cells differentiated in RHB-A culture medium express neural lineage markers, including microtubule associate protein 2 (Panel B), neuron-specific class III beta-tubulin (Panel C), gamma amino butyric acid (Panel D), and glial fibrillary acidic protein (Panel E)
Adherent human neural stem cells differentiated in RHB-A culture medium express neural lineage markers, including microtubule associate protein 2 (Panel B), neuron-specific class III beta-tubulin (Panel C), gamma amino butyric acid (Panel D), and glial fibrillary acidic protein (Panel E). A phase contrast image (Panel A) and a merge of neuron-specific class III beta-tubulin and glial fibrillary acidic protein images (F) are also shown.
Adherent human neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers
Adherent human neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers. Expression of nestin (Panel A), vimentin (Panel B), 3CB2 (Panel C), and microtubule associated protein 2 (Panel D) were assessed by immunofluorescence.
Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers, including Nestin, Vimentin (3CB2), radial glial cell marker-2 (RC2), glial fibrillary acidic protein (GFAP), and microtubule associate protein (MAP)
Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers, including Nestin, Vimentin (3CB2), radial glial cell marker-2 (RC2), glial fibrillary acidic protein (GFAP), and microtubule associate protein (MAP).
Human neural cortex stem cell lines were differentiated into either neurons (MAP2, red) or astrocytes (GFAP, green) using RHB-A
Human neural cortex stem cell lines were differentiated into either neurons (MAP2, red) or astrocytes (GFAP, green) using RHB-A.
Fold expansion profiles of human neural stem cell lines cultured by different vendor’s systems
Fold expansion profiles of human neural stem cell lines cultured by different vendor’s systems. RHB-A exhibits robust growth and expansion of human neural stem cells.
Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express sex determining region Y-box 2 (Sox2, red) and Nestin (green)
Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express sex determining region Y-box 2 (Sox2, red) and Nestin (green).
Abranches, E. et al. Neural Differentiation of Embryonic Stem Cells In Vitro: A Road Map to Neurogenesis in the Embryo. PLoS One 4, e6286 (2009).
Conti, L. et al. Niche-Independent Symmetrical Self-Renewal of a Mammalian Tissue Stem Cell. PLoS Biol. 3, e283 (2005).
Diogo, M. M., Henrique, D. & Cabral, J. M. S. Optimization and integration of expansion and neural commitment of mouse embryonic stem cells. Biotechnol. Appl. Biochem. 49, 105 (2008).
Hansen, D. V., Lui, J. H., Parker, P. R. L. & Kriegstein, A. R. Neurogenic radial glia in the outer subventricular zone of human neocortex. Nature 464, 554–561 (2010).
Sun, Y. et al. Long-term tripotent differentiation capacity of human neural stem (NS) cells in adherent culture. Mol. Cell. Neurosci. 38, 245–258 (2008).
Ying, Q. L., Stavridis, M., Griffiths, D., Li, M. & Smith, A. Conversion of embryonic stem cells into neuroectodermal precursors in adherent monoculture. Nat. Biotechnol. 21, 183–186 (2003).
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Adherent human neural stem cells differentiated in RHB-A culture medium express neural lineage markers, including microtubule associate protein 2 (Panel B), neuron-specific class III beta-tubulin (Panel C), gamma amino butyric acid (Panel D), and glial fibrillary acidic protein (Panel E)
Adherent human neural stem cells differentiated in RHB-A culture medium express neural lineage markers, including microtubule associate protein 2 (Panel B), neuron-specific class III beta-tubulin (Panel C), gamma amino butyric acid (Panel D), and glial fibrillary acidic protein (Panel E). A phase contrast image (Panel A) and a merge of neuron-specific class III beta-tubulin and glial fibrillary acidic protein images (F) are also shown.
Adherent human neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers
Adherent human neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers. Expression of nestin (Panel A), vimentin (Panel B), 3CB2 (Panel C), and microtubule associated protein 2 (Panel D) were assessed by immunofluorescence.
Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers, including Nestin, Vimentin (3CB2), radial glial cell marker-2 (RC2), glial fibrillary acidic protein (GFAP), and microtubule associate protein (MAP)
Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express neural stem cell markers, including Nestin, Vimentin (3CB2), radial glial cell marker-2 (RC2), glial fibrillary acidic protein (GFAP), and microtubule associate protein (MAP).
Human neural cortex stem cell lines were differentiated into either neurons (MAP2, red) or astrocytes (GFAP, green) using RHB-A
Human neural cortex stem cell lines were differentiated into either neurons (MAP2, red) or astrocytes (GFAP, green) using RHB-A.
Fold expansion profiles of human neural stem cell lines cultured by different vendor’s systems
Fold expansion profiles of human neural stem cell lines cultured by different vendor’s systems. RHB-A exhibits robust growth and expansion of human neural stem cells.
Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express sex determining region Y-box 2 (Sox2, red) and Nestin (green)
Adherent mouse neural stem cells cultured in RHB-A medium supplemented with epidermal growth factor and fibroblast growth factor-2 express sex determining region Y-box 2 (Sox2, red) and Nestin (green).
