RetroNectin ELISA Kit
RetroNectin ELISA Kit
Brand: Takara Bio.
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RetroNectin ELISA Kit
RetroNectin detection
This kit is a sandwich ELISA using as the capture antibody a plate-coated RetroNectin-specific monoclonal antibody that does not cross-react with human fibronectin. It is not subject to inhibition even in the presence of human blood components, allowing easy monitoring of the level of RetroNectin. This kit allows highly sensitive quantitative determination of release from RetroNectin-coated plates and residual RetroNectin levels in both cryopreservation solutions of cells cultured in a RetroNectin-added medium and cell washings.
Applications
- Quantitation of RetroNectin in cryopreservation solutions, cell culture fluids, and washes
- Sensitive detection of as little as 3.1 ng/ml of RetroNectin
Performance
- Range of assay: 3.1–200 ng/ml
- Sensitivity: 3.1 ng/ml
- Assay duration: 2.5 hours
- Specificity: RetroNectin
- Test specimen type: Cryopreservation solutions, cell culture fluids, and washes.
- Specimen volume required: 100 µl
Product citations
Donahue, R.E. et al. Fibronectin fragment CH-296 inhibits apoptosis and enhances ex vivo gene transfer by murine retrovirus and human lentivirus vectors independent of viral tropism in nonhuman primate CD34+ cells. Mol. Ther. 3, 359–367 (2001).
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