Retro-X Universal Packaging System
Retro-X Universal Packaging System
Brand: Takara Bio.
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Retro-X Universal Packaging System
This kit provides a complete retroviral vector packaging system for use with any retroviral vector. It is designed to produce virus that can infect dividing cells from a broad range of mammalian and nonmammalian cell types. The GP2-293 Packaging Cell Line contains only the MoMuLV gag and pol genes. The viral envelope portion of the packaging function (env gene) is supplied by transiently co-transfecting one of the Retro-X Universal Packaging Vectors (p10A1, pAmpho, pEco, or pVSV-G) with the retroviral vector. The pQCLIN Retroviral Vector (included in the vector set) can be used as a positive control to constitutively express β-galactosidase in transduced mammalian cells.
Overview
- We recommend the Retro-X Universal Packaging System for most applications
- This system produces the highest titers
- GP2-293 cells are easy to transfect which leads to very high titers
- It is supplied with all four commonly used envelopes for great flexibility (including VSV-G and Eco)
- Retrovirus is produced by transient transfection of the envelope vector and retrovirus vector
- Use RetroPack cells if you wish to create a stable producer cell line
- All retroviral packaging cell lines and systems are compatible with all retroviral vectors sold by Takara Bio, including all MMLV- and MSCV-based retroviruses.
Schematic for retroviral packaging using Retro-X Universal Packaging System
Schematic for retroviral packaging using Retro-X Universal Packaging System.
Retrovirus packaging options from Clontech
Retrovirus packaging options from Clontech. Summary of the cell lines and tropisms associated with commonly used retroviral envelopes.
Characteristics of retroviral packaging cell lines: AmphoPack-293, EcoPack 2-293, and RetroPack PT67
Characteristics of retroviral packaging cell lines: AmphoPack-293, EcoPack 2-293, and RetroPack PT67.
High titer retroviral packaging with a choice of four different retroviral packaging envelopes
High titer retroviral packaging with a choice of four different retroviral packaging envelopes. GP2-293 Cells were transfected with a Retro-X Q vector expressing the red fluorescent protein DsRed2, and each of the four envelope vectors supplied with the Retro-X Unversal Packaging System. After incubating the transfected GP2-293 cells for 48 hr, 10 ?l of the resulting virus was used to infect NIH 3T3 cells. After 48 hours, titers were measured by flow cytometry. Titers were calculated by multiplying the percent infected cells (infectious units) by the total cell number, then dividing by the volume of virus (ml). Panel A. Ecotropic envelope. Panel B. Amphotropic envelope. Panel C. Dualtropic envelope. Panel D. VSV-G envelope (pantropic). Positive cells are indicated by the red line. Negative control cells are indicated by the green line.
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Schematic for retroviral packaging using Retro-X Universal Packaging System
Schematic for retroviral packaging using Retro-X Universal Packaging System.
Retrovirus packaging options from Clontech
Retrovirus packaging options from Clontech. Summary of the cell lines and tropisms associated with commonly used retroviral envelopes.
Characteristics of retroviral packaging cell lines: AmphoPack-293, EcoPack 2-293, and RetroPack PT67
Characteristics of retroviral packaging cell lines: AmphoPack-293, EcoPack 2-293, and RetroPack PT67.
High titer retroviral packaging with a choice of four different retroviral packaging envelopes
High titer retroviral packaging with a choice of four different retroviral packaging envelopes. GP2-293 Cells were transfected with a Retro-X Q vector expressing the red fluorescent protein DsRed2, and each of the four envelope vectors supplied with the Retro-X Unversal Packaging System. After incubating the transfected GP2-293 cells for 48 hr, 10 ?l of the resulting virus was used to infect NIH 3T3 cells. After 48 hours, titers were measured by flow cytometry. Titers were calculated by multiplying the percent infected cells (infectious units) by the total cell number, then dividing by the volume of virus (ml). Panel A. Ecotropic envelope. Panel B. Amphotropic envelope. Panel C. Dualtropic envelope. Panel D. VSV-G envelope (pantropic). Positive cells are indicated by the red line. Negative control cells are indicated by the green line.
