Retro-X Tet-On 3G Inducible Expression System
Retro-X Tet-On 3G Inducible Expression System
Brand: Takara Bio.
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Retro-X Tet-On 3G Inducible Expression System
The Retro-X Tet-On 3G Inducible Expression System is a tetracycline-inducible, retroviral gene expression system that allows you to produce high titers of recombinant retroviruses for the purpose of establishing a tightly inducible expression system for your gene of interest in a wide variety of dividing mammalian cells. In the presence of doxycycline (Dox), the Tet-On 3G transactivator specifically binds and activates high-level transcription from the inducible promoter that controls expression of your gene.
Overview
- Outstanding induction control and extremely low basal expression for a broad range of dividing cell types
- TRE3G promoter is optimized for very tight transcriptional control
- Supplied with our best-performing retroviral packaging system (Retro-X Universal)
- Tet-One formats are also available
Applications
- Establish a tetracycline-inducible gene expression system in a wide variety of dividing cells using retrovirus
The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox)
The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox). When Dox binds, the transactivator undergoes a conformational change allowing it to bind tet operator (tetO) repeats within the TRE3G promoter.
Vector formats for lentiviral (pLVX) and retroviral (pRetroX) Tet-On 3G tetracycline inducible expression systems
Vector formats for lentiviral (pLVX) and retroviral (pRetroX) Tet-On 3G tetracycline inducible expression systems. Each system includes a regulator vector that stably expresses the Tet-On 3G transactivator protein, and a response vector that contains the PTRE3GV promoter that controls the expression of your transgene.
Tetracycline-inducible expression combined with retroviral delivery
Tetracycline-inducible expression combined with retroviral delivery. MCF7 cells were co-transduced at a 1:1 ratio with Retro-X Tet-On 3G retrovirus and Retro-X-TRE3G-Luc retrovirus engineered for inducible luciferase expression. Transduced cells were grown in the absence or presence of Dox for 48 hr and assayed for luciferase activity. RLU = relative light units.
Inducible expression of luciferase using transduced MMLV retrovirus
Inducible expression of luciferase using transduced MMLV retrovirus. VSV-G pseudotyped RetroX-Tet3G and RetroX-TRE3G-Luc retrovirus were generated with the Retro-X Universal Packaging System (supplied) and used to co-infect HepG2 cells at a 1:1 ratio. After 24 hr, the transduced cells were treated with the indicated dose of doxycycline (Dox). Expression of luciferase was determined a further 48 hr later using an anti-luciferase antibody on a Western blot.
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The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox)
The Tet-On 3G systems allow inducible gene expression only in the presence of doxycycline (Dox). When Dox binds, the transactivator undergoes a conformational change allowing it to bind tet operator (tetO) repeats within the TRE3G promoter.
Vector formats for lentiviral (pLVX) and retroviral (pRetroX) Tet-On 3G tetracycline inducible expression systems
Vector formats for lentiviral (pLVX) and retroviral (pRetroX) Tet-On 3G tetracycline inducible expression systems. Each system includes a regulator vector that stably expresses the Tet-On 3G transactivator protein, and a response vector that contains the PTRE3GV promoter that controls the expression of your transgene.
Tetracycline-inducible expression combined with retroviral delivery
Tetracycline-inducible expression combined with retroviral delivery. MCF7 cells were co-transduced at a 1:1 ratio with Retro-X Tet-On 3G retrovirus and Retro-X-TRE3G-Luc retrovirus engineered for inducible luciferase expression. Transduced cells were grown in the absence or presence of Dox for 48 hr and assayed for luciferase activity. RLU = relative light units.
Inducible expression of luciferase using transduced MMLV retrovirus
Inducible expression of luciferase using transduced MMLV retrovirus. VSV-G pseudotyped RetroX-Tet3G and RetroX-TRE3G-Luc retrovirus were generated with the Retro-X Universal Packaging System (supplied) and used to co-infect HepG2 cells at a 1:1 ratio. After 24 hr, the transduced cells were treated with the indicated dose of doxycycline (Dox). Expression of luciferase was determined a further 48 hr later using an anti-luciferase antibody on a Western blot.
