Retro-X Concentrator Kit
The Retro-X Concentrator is a complete reagent for the concentration of infectious retroviral vector particles. This reagent provides a scalable alternative to ultracentrifugation for viral particle concentration. Vector supernatants can be concentrated 10–100 fold, depending upon the volumes and envelope used.
Overview
- Simple mix-and-spin protocol
- Hassle-free and easily scaled up for large volumes
- No ultracentrifugation required
- 100X concentration
- 90% yield
- Retro-X Concentrator can also concentrate lentivirus supernatants utilizing the incubation times recommended in the Lenti-X Concentrator Protocol-At-A-Glance
Applications
- Retroviral transduction studies
- Increased multiplicity of infection (MOI)
- Rescue of low-titer supernatants
Simple method to concentrate retrovirus
Simple method to concentrate retrovirus. Add Retro-X Concentrator reagent to clarified viral supernatant, incubate overnight at 4°C, and spin. That’s it.
Far simpler than ultracentrifugation
Far simpler than ultracentrifugation. In a side-by-side comparison of Retro-X Concentrator and ultracentrifugation, the advantages of the Retro-X method are clearly evident. Retro-X Concentrator is more flexible and easier to use than ultracentrifugation. The Retro-X Concentrator reagent is itself a 4X concentrate, so it can be added to any volume of supernatant containing any amount of virus or any starting titer. Scalability has been successful for supernatant volumes up to 250 ml.
Retro-X Concentrator can concentrate retroviral supernatants created with all commonly used envelopes
Retro-X Concentrator can concentrate retroviral supernatants created with all commonly used envelopes. A retrovirus expressing DsRed2 was packaged with different retroviral envelopes using the Retro-X Universal Packaging System (Cat. # 631530), and harvested at 72 hr posttransfection. Retro-X Concentrator was used to concentrate dualtropic (10A1), amphotropic (Ampho), ecotropic (Eco), and VSV-G (pantropic) pseudotyped retrovirus samples from 10 ml down to 100 µl (Panels A & B). Crude and concentrated viral stocks were then titrated on HT1080 or NIH 3T3 cells and analyzed via flow cytometry at 72 hr postinfection to determine the percentage of positive cells.
Retroviral vectors available from Clontech
Retroviral vectors available from Takara Bio. Not all vectors are shown. Some retroviral vectors are not sold separately.
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Simple method to concentrate retrovirus
Simple method to concentrate retrovirus. Add Retro-X Concentrator reagent to clarified viral supernatant, incubate overnight at 4°C, and spin. That’s it.
Far simpler than ultracentrifugation
Far simpler than ultracentrifugation. In a side-by-side comparison of Retro-X Concentrator and ultracentrifugation, the advantages of the Retro-X method are clearly evident. Retro-X Concentrator is more flexible and easier to use than ultracentrifugation. The Retro-X Concentrator reagent is itself a 4X concentrate, so it can be added to any volume of supernatant containing any amount of virus or any starting titer. Scalability has been successful for supernatant volumes up to 250 ml.
Retro-X Concentrator can concentrate retroviral supernatants created with all commonly used envelopes
Retro-X Concentrator can concentrate retroviral supernatants created with all commonly used envelopes. A retrovirus expressing DsRed2 was packaged with different retroviral envelopes using the Retro-X Universal Packaging System (Cat. # 631530), and harvested at 72 hr posttransfection. Retro-X Concentrator was used to concentrate dualtropic (10A1), amphotropic (Ampho), ecotropic (Eco), and VSV-G (pantropic) pseudotyped retrovirus samples from 10 ml down to 100 µl (Panels A & B). Crude and concentrated viral stocks were then titrated on HT1080 or NIH 3T3 cells and analyzed via flow cytometry at 72 hr postinfection to determine the percentage of positive cells.
Retroviral vectors available from Clontech
Retroviral vectors available from Takara Bio. Not all vectors are shown. Some retroviral vectors are not sold separately.
