Refolding CA Kit
Refolding CA Kit
Brand: Takara Bio.
In stock
SKU
Refolding Cycloamylose (CA) Kit
Overview
The Refolding CA Kit uses a novel artificial chaperone technology to allow an easy two-step procedure for determining optimal refolding conditions for inclusion body proteins. Choosing and performing the optimal conditions results in correct protein folding and restoration of protein activity. The small kit (Cat. # 7350) is supplied with guanidine hydrochloride and DTT for protein unfolding, four different surfactants that can be added independently to the unfolded protein solution to provide protection against molecular aggregation, and highly polymerized cycloamylose (CA), an artificial chaperone, for surfactant removal and recovery of protein activity. Overnight incubation of the CA-treated protein is followed by a quick 10-minute centrifugation and collection of the supernatant containing the refolded protein. Use the large kit (Cat. # 7351) for large scale refolding after the reaction conditions have been determined using the small Refolding CA kit. The large kit consists of only denaturant and CA.
Applications
- Refolding of isolated inclusion body proteins
- Solubilization of inclusion body proteins
- Recovery of protein activity
Protocol for Refolding CA Kit
Protocol for Refolding CA Kit.
Principle of Refolding CA Kit
Principle of Refolding CA Kit.
Daugherty, D. L., Rozema, D., Hanson, P. E. & Gellman, S. H. Artificial chaperone-assisted refolding of citrate synthase. J. Biol. Chem. 273, 33961–71 (1998).
Machida, S. et al. Cycloamylose as an efficient artificial chaperone for protein refolding. FEBS Lett. 486, 131–5 (2000).
Sivakama Sundari, C., Raman, B. & Balasubramanian, D. Artificial chaperoning of insulin, human carbonic anhydrase and hen egg lysozyme using linear dextrin chains--a sweet route to the native state of globular proteins. FEBS Lett. 443, 215–9 (1999).
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Protocol for Refolding CA Kit
Protocol for Refolding CA Kit.
Principle of Refolding CA Kit
Principle of Refolding CA Kit.
