Ecotropic Receptor Booster
Ecotropic Receptor Booster
Brand: Takara Bio.
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Ecotropic Receptor Booster
The Ecotropic Receptor Booster increases the transduction efficiency of cells that are either resistant to infection, or inefficiently infected by ecotropic pseudotyped lentivirus or retrovirus. The Ecotropic Receptor Booster consists of exosome-like particles coated with the mCAT-1 receptor. When applied to target cells, fusion of the exosome-like particles with the host cell plasma membrane temporarily increases surface mCAT-1 receptor, thus allowing for efficient viral transduction.The Ecotropic Receptor Booster renders cells that cannot usually be transduced with ecotropic psuedotyped virus (e.g. human celllines) susceptible to viral infection.
Overview
- Increase efficiency of transduction by temporarily increasing the concentration of viral receptor proteins on your target cells
- Simply preincubate your target cells with these concentrated exosome-like vesicles for 2 hours prior to infection with virus
- Infect human cells with ecotropic retrovirus or ecotropic lentivirus
- The Ecotropic Receptor Booster (Cat. # 631471) delivers the mCAT-1 receptor to target cells
Applications
- Increased transduction efficiency of hard-to-infect cell types
- Transduce human cells with ecotropic retrovirus or lentivirus
Receptor boosters are concentrated exosome-like vesicles that are applied to your target cells prior to infection with virus
Receptor boosters are concentrated exosome-like vesicles that are applied to your target cells prior to infection with virus. Boosters result in increased transduction efficiency because treatment increases the cell surface density of the receptor recognized by the lentivirus (ecotropic) or retrovirus (ecotropic or amphotropic). Using this technology, for example, you can coat human cells with the ecotropic receptor (which is otherwise absent), enabling them to be transduced with ecotropic retrovirus or ecotropic lentivirus.
Transduction of human cells with ecotropic lentivirus can result in high infection efficiency, regardless of the cell line used
Transduction of human cells with ecotropic lentivirus can result in high infection efficiency, regardless of the cell line used. Panel A. HT1080 cells were plated in 6-well plates 24 hr prior to transduction and incubated with 10 µl Ecotropic Receptor Booster for 2 hr. Cells were then transduced with Lenti-X ZsGreen1 lentivirus (MOI=~15) produced using Clontech's Lenti-X HTX Ecotropic Packaging System and assayed 48 hr later for ZsGreen1 expression. Panel B. A variety of human cell types that are normally resistant to transduction by ecotropic-pseudotyped lentiviral vectors were rendered susceptible to transduction by pre-treatment with the Ecotropic Receptor Booster.
Usually, it is not possible to infect Jurkat cells, human mesenchymal stem cells, or human primary neural progenitor cells with ecotropic pseudotyped lentivirus
Usually, it is not possible to infect Jurkat cells, human mesenchymal stem cells, or human primary neural progenitor cells with ecotropic pseudotyped lentivirus. However, pre-treatment of these cell types with Ecotropic Receptor Booster allows for very efficient transduction.
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Receptor boosters are concentrated exosome-like vesicles that are applied to your target cells prior to infection with virus
Receptor boosters are concentrated exosome-like vesicles that are applied to your target cells prior to infection with virus. Boosters result in increased transduction efficiency because treatment increases the cell surface density of the receptor recognized by the lentivirus (ecotropic) or retrovirus (ecotropic or amphotropic). Using this technology, for example, you can coat human cells with the ecotropic receptor (which is otherwise absent), enabling them to be transduced with ecotropic retrovirus or ecotropic lentivirus.
Transduction of human cells with ecotropic lentivirus can result in high infection efficiency, regardless of the cell line used
Transduction of human cells with ecotropic lentivirus can result in high infection efficiency, regardless of the cell line used. Panel A. HT1080 cells were plated in 6-well plates 24 hr prior to transduction and incubated with 10 µl Ecotropic Receptor Booster for 2 hr. Cells were then transduced with Lenti-X ZsGreen1 lentivirus (MOI=~15) produced using Clontech's Lenti-X HTX Ecotropic Packaging System and assayed 48 hr later for ZsGreen1 expression. Panel B. A variety of human cell types that are normally resistant to transduction by ecotropic-pseudotyped lentiviral vectors were rendered susceptible to transduction by pre-treatment with the Ecotropic Receptor Booster.
Usually, it is not possible to infect Jurkat cells, human mesenchymal stem cells, or human primary neural progenitor cells with ecotropic pseudotyped lentivirus
Usually, it is not possible to infect Jurkat cells, human mesenchymal stem cells, or human primary neural progenitor cells with ecotropic pseudotyped lentivirus. However, pre-treatment of these cell types with Ecotropic Receptor Booster allows for very efficient transduction.
