plRES2-ZsGreen1 Vector
plRES2-ZsGreen1 Vector
Brand: Takara Bio.
In stock
SKU
plRES2-ZsGreen1 Vector
pIRES2-ZsGreen1 is a bicistronic expression vector containing ZsGreen1 as a marker for transfection efficiency. It encodes a human-codon-optimized variant of the Zoanthus sp. green fluorescent protein, ZsGreen, which has been engineered for brighter fluorescence and higher expression in mammalian cells. A gene of interest is inserted into the MCS located upstream of the encephalomyocarditis virus (ECMV) internal ribosomal entry site (IRES). The IRES sequence allows the gene of interest and ZsGreen1 to be translated simultaneously from the same mRNA transcript.
Use of AcGFP1 for fusions and fluorescence microscopy applications
Use of AcGFP1 for fusions and fluorescence microscopy applications. Panels A and B. Activation of Protein Kinase C alpha was monitored with Living Colors AcGFP1. Panel A. HEK 293 cells were stably transfected with a plasmid encoding AcGFP1 fused to PKC alpha. Panel B. Cells were induced with 1.5 µg/ml PMA for 3 min. The PKC alpha-AcGFP1 fusion moves from the cytosol to the plasma membrane, a result consistent with the known mobilization pattern of PKC alpha. Panel C. HeLa cells were transiently transfected with pAcGFP1-Actin and visualized by fluorescence microscopy.
Map of the fluorescent pIRES2 bicistronic expression vectors
Map of the fluorescent pIRES2 bicistronic expression vectors.
Expression of two proteins from a single mRNA transcript
Expression of two proteins from a single mRNA transcript. A fluorescent protein is translated from an internal ribosome entry site (IRES).
Write Your Own Review
Use of AcGFP1 for fusions and fluorescence microscopy applications
Use of AcGFP1 for fusions and fluorescence microscopy applications. Panels A and B. Activation of Protein Kinase C alpha was monitored with Living Colors AcGFP1. Panel A. HEK 293 cells were stably transfected with a plasmid encoding AcGFP1 fused to PKC alpha. Panel B. Cells were induced with 1.5 µg/ml PMA for 3 min. The PKC alpha-AcGFP1 fusion moves from the cytosol to the plasma membrane, a result consistent with the known mobilization pattern of PKC alpha. Panel C. HeLa cells were transiently transfected with pAcGFP1-Actin and visualized by fluorescence microscopy.
Map of the fluorescent pIRES2 bicistronic expression vectors
Map of the fluorescent pIRES2 bicistronic expression vectors.
Expression of two proteins from a single mRNA transcript
Expression of two proteins from a single mRNA transcript. A fluorescent protein is translated from an internal ribosome entry site (IRES).
