Affinity purified recombinant Discosoma sp. red fluorescent protein (rDsRed2). rDsRed2 can be used as a standard for protein gels, Western blots, fluorometry, and fluorescence activated cell sorting (FACS). It can also be used as a control for DsRed2 expression studies and for microinjection into cells and tissues. At its N-terminus, rDsRed2 contains a 6xHN epitope tag, used for purification of the protein by metal ion affinity chromatagraphy (IMAC) with our TALON Resin. An enterokinase (EK) site, located between the tag and DsRed2, makes it possible to remove the 6xHN polypetide if desired. The 6xHN tag does not interfere with the protein's characteristic spectral properties.
Overview
Purified, recombinant proteins from E. coli
Protein retains the absorption and emission spectra identical to expressed proteins
Retains fluorescence capability under harsh conditions
Applications
Western blotting
Calibration of fluorescent protein detection instrumentation
Affinity purified recombinant Discosoma sp. red fluorescent protein (rDsRed2). rDsRed2 can be used as a standard for protein gels, Western blots, fluorometry, and fluorescence activated cell sorting (FACS). It can also be used as a control for DsRed2 expression studies and for microinjection into cells and tissues. At its N-terminus, rDsRed2 contains a 6xHN epitope tag, used for purification of the protein by metal ion affinity chromatagraphy (IMAC) with our TALON Resin. An enterokinase (EK) site, located between the tag and DsRed2, makes it possible to remove the 6xHN polypetide if desired. The 6xHN tag does not interfere with the protein's characteristic spectral properties.
Overview
Purified, recombinant proteins from E. coli
Protein retains the absorption and emission spectra identical to expressed proteins
Retains fluorescence capability under harsh conditions
Applications
Western blotting
Calibration of fluorescent protein detection instrumentation
