Rat Universal Reference Total RNA
Rat Universal Reference Total RNA
Brand: Takara Bio.
In stock
SKU
Rat Universal Reference Total RNA
Reference RNA for real-time qPCR
Mixture of total RNAs from a collection of adult rat tissues, chosen to represent a broad range
of expressed genes. Both male and female animals are represented. Rat Universal Reference Total RNA serves as a standard for the comparison of gene expression data and is useful as an internal control for microarray expression profiling experiments.
Total RNA was isolated by a modified guanidinium thiocyanate method.
Overview
- Featuring the broadest possible gene representation with minimal lot-to-lot variation
- Use with any microarray or labeling method
- Less gene-to-gene signal variation
- Virtually free of genomic DNA
Applications
- Microarray analysis
- Real-time PCR/qPCR
- RT-PCR
- Northern analysis
- Gene-expression analysis
Clontech Rat Universal Reference Total RNA demonstrates more than 90% gene coverage
Clontech Rat Universal Reference Total RNA demonstrates more than 90% gene coverage. Cy3 labeled probes were generated using our Rat Reference Total RNA (Row A), another vendor’s reference total RNA (Row B), a random RNA mixture (Row C), and an individual RNA sample (Row D). Probes were hybridized to a glass rat array containing 3,766 long oligonucleotides. Expression results were analyzed using GeneSpring software (version 5.0) to cluster genes according to their expression patterns. Varying colors reflect the ratio of the intensity of any gene on each array to its median intensity across all arrays. The red and blue colors reflect high and low ratios, respectively. Our results indicate that the Reference Total RNA has more than 90% gene coverage with even distribution and outperforms another vendor’s universal RNA mixture.
Write Your Own Review
Clontech Rat Universal Reference Total RNA demonstrates more than 90% gene coverage
Clontech Rat Universal Reference Total RNA demonstrates more than 90% gene coverage. Cy3 labeled probes were generated using our Rat Reference Total RNA (Row A), another vendor’s reference total RNA (Row B), a random RNA mixture (Row C), and an individual RNA sample (Row D). Probes were hybridized to a glass rat array containing 3,766 long oligonucleotides. Expression results were analyzed using GeneSpring software (version 5.0) to cluster genes according to their expression patterns. Varying colors reflect the ratio of the intensity of any gene on each array to its median intensity across all arrays. The red and blue colors reflect high and low ratios, respectively. Our results indicate that the Reference Total RNA has more than 90% gene coverage with even distribution and outperforms another vendor’s universal RNA mixture.
