Marathon-Ready cDNA is high-quality, double-stranded cDNA which has been ligated to the Marathon Adaptor and is ready for use as a template in 5' and 3' Marathon RACE reactions. In many cases, the full-length cDNA can then be obtained by end-to-end amplification or standard cloning. Enough material is provided for 30 50-μl Marathon RACE reactions.
Overview
Adaptor-ligated and ready for RACE PCR
Tissue-specific "pool" of double-stranded cDNA
Obtain full-length cDNAs without ever constructing or screening a cDNA library
Perfect template for Marathon cDNA Amplification
Applications
Rapid amplification of cDNA ends (RACE)
Study tissue-specific gene expression
Discover polymorphic forms of mRNA
Obtain full-length copies of published cDNAs
Characterization of tissue-specific patterns of gene expression
Marathon RACE products obtained using G3PDH primers to amplify Marathon-Ready Human, Mouse, and Rat Heart cDNAs
Marathon RACE products obtained using G3PDH primers to amplify Marathon-Ready Human, Mouse, and Rat Heart cDNAs. RACE PCR was performed using the AP1 primer and the positive control G3PDH primers. The templates used were Marathon-Ready Human Heart (Cat. # 639304), Mouse Heart (Cat. # 639404), and Rat Heart (Cat. # 639416) cDNAs. Lane M: 1-kb DNA size ladder.
Borson, N. D., et al. A lock-docking oligo(dT) primer for 5' and 3' RACE PCR. PCR Methods Appl. 2, 144–148 (1992).
Chenchik, A., et al. Full-Length cDNA Cloning and Determination of mRNA 5' and 3' Ends by Amplification of Adaptor-Ligated cDNA. BioTechniques 21, 526–532 (1996).
Marathon-Ready cDNA is high-quality, double-stranded cDNA which has been ligated to the Marathon Adaptor and is ready for use as a template in 5' and 3' Marathon RACE reactions. In many cases, the full-length cDNA can then be obtained by end-to-end amplification or standard cloning. Enough material is provided for 30 50-μl Marathon RACE reactions.
Overview
Adaptor-ligated and ready for RACE PCR
Tissue-specific "pool" of double-stranded cDNA
Obtain full-length cDNAs without ever constructing or screening a cDNA library
Perfect template for Marathon cDNA Amplification
Applications
Rapid amplification of cDNA ends (RACE)
Study tissue-specific gene expression
Discover polymorphic forms of mRNA
Obtain full-length copies of published cDNAs
Characterization of tissue-specific patterns of gene expression
Marathon RACE products obtained using G3PDH primers to amplify Marathon-Ready Human, Mouse, and Rat Heart cDNAs
Marathon RACE products obtained using G3PDH primers to amplify Marathon-Ready Human, Mouse, and Rat Heart cDNAs. RACE PCR was performed using the AP1 primer and the positive control G3PDH primers. The templates used were Marathon-Ready Human Heart (Cat. # 639304), Mouse Heart (Cat. # 639404), and Rat Heart (Cat. # 639416) cDNAs. Lane M: 1-kb DNA size ladder.
Borson, N. D., et al. A lock-docking oligo(dT) primer for 5' and 3' RACE PCR. PCR Methods Appl. 2, 144–148 (1992).
Chenchik, A., et al. Full-Length cDNA Cloning and Determination of mRNA 5' and 3' Ends by Amplification of Adaptor-Ligated cDNA. BioTechniques 21, 526–532 (1996).