Random Primer DNA Labeling
Random Primer DNA Labeling
Brand: Takara Bio.
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Random Primer DNA Labeling
Random Primer DNA Labeling
The Random Primer DNA Labeling Kit, Version 2 is designed to yield DNA probes with high activity for hybridization and can be used to label DNA with [32P]-α-, [35S]-α- or [3H]-α-dCTP. This kit is based on a modified method of Feinberg and Vogelstein that utilizes random oligonucleotide primers and the cloned exonuclease-free Klenow fragment of E. coli DNA polymerase I. The use of longer 9-mer primers and exonuclease-free enzyme results in higher labeling efficiency and longer probes. This method overcomes many of the disadvantages of the conventional nick translation procedure while producing probes from small amounts of DNA (10 to 20 ng). It can also be used to label DNA fragments that are embedded in low-melting temperature agarose gel slices.
Applications
- Probe labeling
- Random primer DNA labeling
References
Feinberg, A. P. & Vogelstein, B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Anal. Biochem. 132, 6–13 (1983).
Feinberg, A. P. & Vogelstein, B. A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity. Addendum. Anal. Biochem. 137, 266–7 (1984).
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