qPCR Human Reference Total RNA
qPCR Human Reference Total RNA
Brand: Takara Bio.
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qPCR Human Reference Total RNA
Reference RNA for real-time qPCR
A mixture of total RNAs from a collection of adult human tissues, chosen to represent a broad range of expressed genes. Both male and female donors are represented.
qPCR Reference Total RNA serves as a standard for the accurate and reproducible comparison of gene expression data using real-time quantitative PCR (qPCR). It may also be used as a source of positive control templates for validating qPCR primer designs.
Total RNA was isolated by a modified guanidinium thiocyanate method.
Overview
- Featuring the broadest possible gene representation with minimal lot-to-lot variation
- Use with any microarray or labeling method
- Less gene-to-gene signal variation
- Virtually free of genomic DNA
Applications
- Microarray analysis
- Real-time PCR/qPCR
- RT-PCR
- Northern analysis
- Gene-expression analysis
High-, medium- and low-abundance gene targets are easily detected in qPCR Human Reference cDNA
High-, medium- and low-abundance gene targets are easily detected in qPCR Human Reference cDNA. Oligo dT-primed (Panels A–C) and random oligo-primed (Panels D–F) qPCR Human Reference cDNA samples were each serially diluted fivefold such that the final quantities of cDNA template in the qPCR reactions were 20 ng, 4 ng, 800 pg, 160 pg, 32 pg,and 6.4 pg. Data were obtained on a Stratagene Mx3000P real-time PCR instrument. The primer sets for all three genes demonstrate the ability to detect a wide range of signals from the serially diluted samples. PPIA = peptidylprolyl isomerase A (cyclophilin A; high-abundance); HPRT = hypoxanthine phosphoribosyltransferase I (medium-abundance); PBGD = porphobilinogen deaminase (low-abundance). Slope and R2 values refer to the line determinedby plotting Ct values versus template quantity
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High-, medium- and low-abundance gene targets are easily detected in qPCR Human Reference cDNA
High-, medium- and low-abundance gene targets are easily detected in qPCR Human Reference cDNA. Oligo dT-primed (Panels A–C) and random oligo-primed (Panels D–F) qPCR Human Reference cDNA samples were each serially diluted fivefold such that the final quantities of cDNA template in the qPCR reactions were 20 ng, 4 ng, 800 pg, 160 pg, 32 pg,and 6.4 pg. Data were obtained on a Stratagene Mx3000P real-time PCR instrument. The primer sets for all three genes demonstrate the ability to detect a wide range of signals from the serially diluted samples. PPIA = peptidylprolyl isomerase A (cyclophilin A; high-abundance); HPRT = hypoxanthine phosphoribosyltransferase I (medium-abundance); PBGD = porphobilinogen deaminase (low-abundance). Slope and R2 values refer to the line determinedby plotting Ct values versus template quantity
